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| Status |
Public on Sep 25, 2024 |
| Title |
Transcriptomic analysis of bacterial vaginosis-associated bacteria living in single or multispecies biofilms |
| Organisms |
Gardnerella vaginalis; Prevotella bivia; Fannyhessea vaginae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bacterial vaginosis (BV) is the most common cause of vaginal discharge among women worldwide. BV is characterized by an imbalance in the vaginal microbiota with depletion of protective Lactobacillus species and overgrowth of facultative and strictly anaerobic bacteria. Although the development of a polymicrobial biofilm on the vaginal epithelium is a hallmark of BV, interactions between key BV-associated bacteria (BVAB) [i.e. Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia] present in the biofilm are still not completely understood. In this study, we aimed to analyse the transcriptome of single and triple-species biofilms growing in the rich medium, New York City III (NYCIII). A previous analysis of triple-species biofilms composition by qPCR showed that the biofilms were mainly composed of G. vaginalis, followed by F. vaginae and P. bivia. The transcriptomic analysis revealed a total of 431 (34 upregulated and 397 downregulated), 126 (36 upregulated and 90 downregulated), and 39 (31 upregulated and 8 downregulated) differentially expressed genes for G. vaginalis, F. vaginae, and P. bivia, respectively. Gene ontology only detected enrichment for the downregulated genes of G. vaginalis and 47 GO terms were associated with molecular functions, cellular components and biological processes, mainly metabolism. Hence, this work showed the adaptation of 3 BVAB when growing in a triple-species biofilm, with several genes being differentially expressed in all the species growing in a polymicrobial biofilm.
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| Overall design |
To study the alterations that growing in single or in multispecies biofilms may have on bacterial vaginosis-associated bacteria, G. vaginalis, F. vaginae and P. bivia were grown both in single and triple-species biofilms and their transcriptomes were characterised.
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| Contributor(s) |
Sousa LG, Novak J, França A, Muzny CA, Cerca N |
| Citation(s) |
39294302 |
| |
| Submission date |
May 22, 2024 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Ângela França |
| E-mail(s) |
angelamosf@gmail.com
|
| Organization name |
University of Minho
|
| Department |
Biological Engineering
|
| Street address |
Campus de Gualtar
|
| City |
Braga |
| ZIP/Postal code |
4710-057 |
| Country |
Portugal |
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| Platforms (4)
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| GPL34514 |
NextSeq 2000 (Gardnerella vaginalis) |
| GPL34515 |
NextSeq 2000 (Fannyhessea vaginae) |
| GPL34516 |
NextSeq 2000 (Prevotella bivia) |
| GPL34517 |
NextSeq 2000 (Fannyhessea vaginae; Gardnerella vaginalis; Prevotella bivia) |
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| Samples (12)
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| GSM8285957 |
Gardnerella vaginalis single biofilms_biol rep1 (br1) |
| GSM8285958 |
Gardnerella vaginalis single biofilms_biol rep2 (br2) |
| GSM8285959 |
Gardnerella vaginalis single biofilms_biol rep3 (br3) |
| GSM8285960 |
Fannyhessea vaginae single biofilms_biol rep1 (br1) |
| GSM8285961 |
Fannyhessea vaginae single biofilms_biol rep2 (br2) |
| GSM8285962 |
Fannyhessea vaginae single biofilms_biol rep3 (br3) |
| GSM8285963 |
Prevotella bivia single biofilms_biol rep1 (br1) |
| GSM8285964 |
Prevotella bivia single biofilms_biol rep2 (br2) |
| GSM8285965 |
Prevotella bivia single biofilms_biol rep3 (br3) |
| GSM8285966 |
Mixed three-species biofilms_biol rep1 (br1) |
| GSM8285967 |
Mixed three-species biofilms_biol rep2 (br2) |
| GSM8285968 |
Mixed three-species biofilms_biol rep3 (br3) |
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| Relations |
| BioProject |
PRJNA1114702 |
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