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Series GSE26737 Query DataSets for GSE26737
Status Public on Feb 01, 2012
Title Epigenetic Transgenerational Alterations to Stress Response in Brain Gene Networks and Behaviour
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Ancestral environmental exposures that promote epigenetic transgenerational inheritance influence all aspects of an individual’s life history. Stress experienced during adolescence can affect adult physiological and behavioural phenotypes. The current study utilized a systems biology approach to investigate the interactions of these two forms of epigenetic modification, one carried in the germline transgenerationally and the other contained in the context of life history. A transgenerational epigenetic imprint left by the fungicide vinclozolin promoted regional specific brain gene networks that influenced chronic restraint stress responses to alter adult physiological, brain and behavioural phenotypes. The environmentally-induced epigenetic transgenerational inheritance was found to interact with early life stress response to impact the adult brain genome activity to bring “the phenotype into being”.
We used microarrays to determine genes expressed differentially in rats' 4 brain areas - basolateral amygdala (BLA), primary and secondary motor cortex (Crtx), CA1 of the hippocampus (CA1), and CA3 of the hippocampus (CA3) - due to Vinclozolin treatments of their grand-grandmothers and/or to stress in adolescence age.
 
Overall design For each of 4 brain areas (BLA, CRTX, CA1, and CA3), RNA samples from 4 treatment groups - stressed control (St-Con), non-stressed control (NSt-Con), stressed vinclozolin (St-Vin), and non-stressed vinclozolin (NSt-Vin) - were compared to each other. Each treatment groups contained 3 biological replica. RNA for each replica was pooled from 4 individual animals forming a certain dyad group.
 
Contributor(s) Crews D, Gillette R, Manikkam M, Savenkova M, Skinner M
Citation(s) 22615374
Submission date Jan 19, 2011
Last update date Nov 06, 2013
Contact name Michael K Skinner
E-mail(s) skinner@mail.wsu.edu
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
 
Platforms (1)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
Samples (48)
GSM658123 Basolateral Amygdala_Non-Stressed_Control_biological rep1
GSM658124 Basolateral Amygdala_Non-Stressed_Control_biological rep2
GSM658125 Basolateral Amygdala_Non-Stressed_Control_biological rep3
Relations
BioProject PRJNA136321

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26737_RAW.tar 207.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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