|
| Status |
Public on Jun 25, 2024 |
| Title |
The primitive endoderm supports lineage plasticity to enable regulative development [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mammalian blastocyst formation involves the specification of trophectoderm followed by differentiation of the inner cell mass into embryonic epiblast and extra-embryonic primitive endoderm (PrE). During this time, the embryo maintains a window of plasticity and can redirect its cellular fate when challenged experimentally. In this context, we found that the PrE alone was sufficient to regenerate a complete blastocyst and continue postimplantation development. We identify an in vitro population similar to the early PrE in vivo that exhibits the same embryonic and extra-embryonic potency and can form complete stem cell-based embryo models termed blastoids. Commitment in the PrE is suppressed by JAK/STAT signalling, collaborating with OCT4 and the sustained expression of a subset of pluripotency-related transcription factors that safeguard an enhancer landscape permissive for multi-lineage differentiation. Our observations support the notion that transcription factor persistence underlies plasticity in regulative development and highlights the importance of the PrE in perturbed development.
|
| |
|
| Overall design |
scRNA-seq of mouse 2iLIF ESCs, naïve extra-embryonic endoderm (nEnd), 3D nEnd and nEnd differentiated is trophoblast stem cell medium (TSC)
|
| |
|
| Contributor(s) |
Linneberg-Agerholm M, Redó-Riveiro A, Charlotte Sell A, Perera M, Proks M, Knudsen TE, Barral A, Manzanares M, Brickman JM |
| Citation(s) |
38917790 |
| |
| Submission date |
May 10, 2024 |
| Last update date |
Sep 24, 2024 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA1110211 |
Less...
More...