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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 17, 2024 |
| Title |
Effect of depletion of PRMT2 on gene expression in the HL-60 acute myeloid leukemia cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Arginine methylation is catalyzed by protein arginine methyltransferases (PRMTs) and is involved in various cellular processes, including cancer development. PRMT2 expression is increased in several cancer types although its role in acute myeloid leukemia (AML) remains unknown. Here, we investigate the role of PRMT2 in a cohort of patients with AML, PRMT2 knockout AML cell lines as well as a Prmt2 knockout mouse model. In patients, low PRMT2 expressors are enriched for inflammatory signatures, including the NF-κB pathway, and show inferior survival. In keeping with a role for PRMT2 in control of inflammatory signaling, bone marrow-derived macrophages from Prmt2 KO mice display increased pro-inflammatory cytokine signaling upon LPS treatment. In PRMT2-depleted AML cell lines, aberrant inflammatory signaling has been linked to overproduction of IL6, resulting from a deregulation of the NF-κB signaling pathway, therefore leading to hyperactivation of STAT3. Together, these findings identify PRMT2 as a key regulator of inflammation in AML.
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| Overall design |
To investigate the functions of PRMT2 in AML, we used the CRISPR-Cas9 editing strategy on the HL-60 cells to generate stable PRMT2 knockout AML cell lines. We performed single-cell sorting on edited cells. Then, PRMT2 gene and protein expressions were screened on all the clones. The best KO clones (named 'AA13' and AA14') were selected and prepared for bulk RNA-sequencing, as well as the wild type (WT) HL-60 cell line as a control. All 3 samples were run on triplicates.
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| Contributor(s) |
Camille S, Romain A, Laurent D, Cyril F |
| Citation(s) |
38902349 |
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| Submission date |
Apr 30, 2024 |
| Last update date |
Aug 19, 2024 |
| Contact name |
Laurent DELVA |
| E-mail(s) |
laurent.delva@u-bourgogne.fr
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| Organization name |
Université de Bourgogne
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| Department |
Inserm U1231 (CTM)
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| Lab |
Epi2THM
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| Street address |
7 boulevard Jeanne d’Arc
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| City |
Dijon |
| ZIP/Postal code |
21000 |
| Country |
France |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| GSM8243836 |
HL-60 PRMT2 KO, AA13 clone, replicate 1 |
| GSM8243837 |
HL-60 PRMT2 KO, AA13 clone, replicate 2 |
| GSM8243838 |
HL-60 PRMT2 KO, AA13 clone, replicate 3 |
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| Relations |
| BioProject |
PRJNA1106447 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE266252_HL-60.KO_vs_WT.DE_analysis.tsv.gz |
5.3 Mb |
(ftp)(http) |
TSV |
| GSE266252_RAW.tar |
3.2 Mb |
(http)(custom) |
TAR (of TAB) |
SRA Run Selector |
| Raw data are available in SRA |
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