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| Status |
Public on Feb 12, 2025 |
| Title |
Sequencing to Explore the Mechanism Network of N-acetyltransferase 10 (NAT10) Promoting Colorectal cancer in human colorectal cancer cell SW620 [ac4CRIP] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
N-acetyltransferase 10 (NAT10), the only known mRNA ac4C “writer,” plays a significant role in several human diseases, including CRC and gastric cancer. As NAT10 can activate or repress important signaling pathways, such as the EMT pathway, DNA damage repair, or apoptosis, by catalyzing the ac4C modification of oncogene mRNA, targeting NAT10 shows great potential for inhibiting cancer cell malignant progression in vitro and in vivo. This complex network of colorectal cancer progression mediated by NAT10 remains to be identified, which can expand cancer therapy options. To identify potential NAT10-regulated ac4C targets, we checked ac4CRIP Sequencing in human colorectal cancer cell SW620.
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| Overall design |
To identify potential NAT10-regulated ac4C targets, we established NAT10 knocking-down cell lines in SW620 by shRNA. Then we checked ac4CRIP Sequencing to find the interaction between NAT10 and the mRNA of its target in NAT10 knocking-down SW620 cells and its control cells.
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| Contributor(s) |
Song D, Tao G, Wang C, Ma M, An Y, Zheng Y |
| Citation(s) |
39905540 |
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| Submission date |
Apr 26, 2024 |
| Last update date |
Feb 13, 2025 |
| Contact name |
Gongcai Tao |
| E-mail(s) |
taogongcai@outlook.com
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| Phone |
18794803049
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| Organization name |
Renmin Hospital of Wuhan University
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| Department |
Department of Gastrointestinal Surgery
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| Lab |
Key Laboratory of Hubei Province for Digestive System Disease
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| Street address |
No. 9, Zhangzhidong Road, Wuchang District
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| City |
Wu Han |
| State/province |
Hu Bei Province |
| ZIP/Postal code |
430061 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| GSM8239111 |
shNAT10, Input, RIP 1 |
| GSM8239112 |
shNAT10, Input, RIP 2 |
| GSM8239113 |
shNAT10, Input, RIP 3 |
| GSM8239114 |
shControl, IP, RIP 1 |
| GSM8239115 |
shControl, IP, RIP 2 |
| GSM8239116 |
shControl, IP, RIP 3 |
| GSM8239117 |
shNAT10, IP, RIP 1 |
| GSM8239118 |
shNAT10, IP, RIP 2 |
| GSM8239119 |
shNAT10, IP, RIP 3 |
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| Relations |
| BioProject |
PRJNA1105099 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE265993_Acetylated_RNA_sites.mRNA.xlsx |
16.8 Mb |
(ftp)(http) |
XLSX |
| GSE265993_RAW.tar |
1.8 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
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