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Series GSE26548 Query DataSets for GSE26548
Status Public on Nov 01, 2011
Title Strigolactone biosynthesis requires the symbiotic GRAS-type transcription factors NSP1 and NSP2
Platform organisms Sinorhizobium meliloti; Medicago sativa; Medicago truncatula
Sample organism Medicago truncatula
Experiment type Expression profiling by array
Summary Legume GRAS-type transcription factors NSP1 and NSP2 are essential for Rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression upon symbiotic signalling. However, legume NSP1 and NSP2 can be functionally replaced by non-legume orthologs; including rice (Oryza sativa) OsNSP1 and OsNSP2. This shows that both proteins are functionally conserved in higher plants, suggesting an ancient function that was conserved during evolution. Here we show that NSP1 and NSP2 are indispensable for strigolactone biosynthesis in the legume Medicago truncatula as well as rice. Mutant nsp1-nsp2 plants hardly produce strigolactones. The lack of strigolactone biosynthesis coincides with strongly reduced DWARF27 expression in both species. Rice and Medicago represent distinct phylogenetic lineages that split ~150 million years ago. Therefore we conclude that regulation of strigolactone biosynthesis by NSP1 and NSP2 is an ancestral function conserved in higher plants. Since strigolactone biosynthesis is highly regulated by environmental conditions like phosphate starvation, NSP1 and NSP2 will be important tools in future studies on the molecular mechanisms by which environmental sensing is translated into regulation of strigolactone biosynthesis. As NSP1 and NSP2 are single copy genes in legumes, it implies that a single protein complex fulfills a dual regulatory function of different downstream targets; symbiotic and non-symbiotic, respectively.
Overall design Three biological replications are used for roots of wild type A17, nsp1 and nsp2 mutant plants
Contributor(s) Liu W, Kohlen W, Lillo A, Op den Camp R, Ivanov S, Hartog M, Limpens E, Jamil M, Yang W, Hooiveld GJ, Charnikhova T, Bouwmeester HJ, Bisseling T, Geurts R
Citation(s) 22039214
Submission date Jan 11, 2011
Last update date Mar 23, 2012
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL4652 [Medicago] Affymetrix Medicago Genome Array
Samples (9)
GSM652751 root, Mtnsp1-1 mutant, replicate 1
GSM652752 root, Mtnsp1-1 mutant, replicate 2
GSM652753 root, Mtnsp1-1 mutant, replicate 3
BioProject PRJNA136629

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26548_RAW.tar 40.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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