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| Status |
Public on Jun 06, 2024 |
| Title |
Effect of depletion of C10orf71 on gene expression in hearts from embryonic mice at E18.5 and adult mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the specific functional and cellular processes that affected by the mC10orf71 deletion, we compared total RNA expression in hearts from E18.5. Gene Ontology (GO) enrichment analysis showed that down-regulated genes were not significantly enriched in any biological process. The 327 genes up-regulated in KO mice were enriched for energy generation, electron transport chain and ATP synthesis, and many of them are encoded by mitochondrial DNA. These changes were likely to be a compensatory response to the impaired morphogenesis and dysfunction of the KO hearts. Then, we performed RNA-seq on hearts of adult WT and KO mice. GO analysis of down-regulated genes identified two categories of biological processes: mRNA processing/splicing and muscle cell differentiation/contraction.
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| Overall design |
We performed gene expression analysis using data obtained from RNA-seq of 4 WT mice and 4 KO mice at two time points
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| Contributor(s) |
Li Y, Li Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 23, 2024 |
| Last update date |
Jun 06, 2024 |
| Contact name |
Yang Li |
| E-mail(s) |
liyanganzhen@126.com
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| Phone |
86+13811892074
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| Organization name |
Beijing Anzhen Hospital
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| Street address |
Anzhen Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (16)
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| GSM8226830 |
WT4, E18.5, heart |
| GSM8226831 |
KO1, E18.5, heart |
| GSM8226832 |
KO2, E18.5, heart |
| GSM8226833 |
KO3, E18.5, heart |
| GSM8226834 |
KO4, E18.5, heart |
| GSM8226835 |
WT1, adult, heart |
| GSM8226836 |
WT2, adult, heart |
| GSM8226837 |
WT3, adult, heart |
| GSM8226838 |
WT4, adult, heart |
| GSM8226839 |
KO1, adult, heart |
| GSM8226840 |
KO2, adult, heart |
| GSM8226841 |
KO3, adult, heart |
| GSM8226842 |
KO4, adult, heart |
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| Relations |
| BioProject |
PRJNA1103880 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE264733_E18.5.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
| GSE264733_adult.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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