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| Status |
Public on Feb 08, 2025 |
| Title |
The SSUP-72/PINN-1 module is required for efficient 3’end processing and coordinates developmental gene expression during exit from diapause in C. elegans [GROseq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Other
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| Summary |
During the exit from the developmental diapause of Caenorhabditis elegans (C. elegans), a network of growth and developmental genes is activated. These genes are organized into operons, where transcriptional termination is uncoupled from mRNA 3’-end processing. Although the Pol II CTD-S2P mark deposited by CDK-12 is unnecessary during embryogenesis, it plays a critical role in the timely expression of most operonic genes by enhancing SL2 trans-splicing at position 2 and above. This process protects mRNA from degradation and prevents termination. Here, a genetic screen has identified the SSUP-72/PINN-1 module as an effective suppressor of CDK-12-induced defects. Our data show that the SSUP-72/PINN-1 module regulates intra-operon termination and affects 3’ pausing genome-wide, coordinating growth and developmental gene expression during post-embryonic development in C. elegans.
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| Overall design |
Global Run-On sequencing (GRO-seq) for CDK-12as (analogue-sensitive) and its suppressive mutation ssup-72[E22K]. Worms were L1 synchronized by hypochlorite treatment and grown 4H in presence of 3MB-PP1 (2 uM) prior to nuclei extraction.
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| Contributor(s) |
Stubbe F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 11, 2024 |
| Last update date |
Feb 10, 2025 |
| Contact name |
François-Xavier Stubbe |
| E-mail(s) |
stubbe3095@gmail.com
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| Organization name |
UNamur
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| Street address |
60 Rue de Bruxelles
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| City |
Namur |
| ZIP/Postal code |
5000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL32326 |
NextSeq 2000 (Caenorhabditis elegans) |
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| Samples (12)
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| GSM8198231 |
whole worm, cdk-12as;ssup-72[E22K],1 |
| GSM8198232 |
whole worm, N2, 2 |
| GSM8198233 |
whole worm, ssup-72[E22K], 2 |
| GSM8198234 |
whole worm, cdk-12as, 2 |
| GSM8198235 |
whole worm, cdk-12as;ssup-72[E22K],2 |
| GSM8198236 |
whole worm, N2, 3 |
| GSM8198237 |
whole worm, ssup-72[E22K], 3 |
| GSM8198238 |
whole worm, cdk-12as, 3 |
| GSM8198239 |
whole worm, cdk-12as;ssup-72[E22K],3 |
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| Relations |
| BioProject |
PRJNA1099204 |
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