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Series GSE26360 Query DataSets for GSE26360
Status Public on Mar 05, 2012
Title Genome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks in mouse embryonic stem cells (expression)
Organism Mus musculus
Experiment type Expression profiling by array
Summary The tumor suppressor p53 regulates the differentiation of embryonic stem (ES) cells upon DNA damage. However, our understanding of this critical tumor suppressive role of p53 in ES cells is limited, mainly because of the lack of molecular mechanism. Here, we report a widespread cross-regulation of p53-mediated DNA damage signaling and the pluripotent gene network in ES cells using chromatin-immunoprecipitation assay-based sequencing (ChIP-seq) and gene expression microarray. Upon DNA damage, p53 directly regulates the transcription of 3644 genes (p<0.005) in mouse ES cells. Genome-wide analysis revealed a dramatic difference between the regulation of p53-activated and -repressed genes. p53 mainly regulates the promoter regions of activated genes, whereas the main regulatory regions for repressed genes reside in distal regions. Among p53-repressed genes, many are pluripotent transcription factors of ES cells, such as Oct4, Nanog, Sox2, Esrrb, c-Myc, n-Myc and Sall4. Strikingly, these transcriptional factors also directly regulate the transcription of the Trp53 gene, highlighting a previously under-estimated transcriptional regulation of p53 in ES cells. Therefore, p53 signaling and ES pluripotent transcriptional networks form an intertwined circuitry. Together, our results provide mechanistic insights into the crosstalk of p53-mediated DNA damage and ES cell "stemness" transcriptional gene networks and shed light on the tumor suppressive function of p53 in ES cells.
 
Overall design The goal of this experiment is to identify the gene expression changes after adriamycin treatment in a p53-dependent manner.
Total six samples: triplicates for untreated mES cells and triplicates for mES cells treated with adriamycin.
 
Contributor(s) Huang J
Citation(s) 22387025
Submission date Dec 29, 2010
Last update date Mar 04, 2019
Contact name Jing Huang
E-mail(s) huangj3@mail.nih.gov
Organization name National Cancer Institute
Lab Cancer Biology and Genetics
Street address 37 Convent Dr. 37/3140
City Bethesda
State/province MD
ZIP/Postal code MD 20892
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (18)
GSM412775 R1E Mock 1
GSM412776 R1E Mock 2
GSM412777 R1E Mock 3
This SubSeries is part of SuperSeries:
GSE26362 Whole-genome study reveals distinct mechanisms used by p53 to regulate activated and repressed genes in embryonic stem cells
Relations
BioProject PRJNA142239

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26360_RAW.tar 70.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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