 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 26, 2024 |
| Title |
Fecal microbiota transfer from autoimmune dry eye disease model mice to conventional mice induces ocular surface inflammation and stereotypic B cell receptor repertoire development in lacrimal gland |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we transferred gut microbiota of SS-like autoimmune dry eye disease model mice to conventional B6 mice (NOD-FMT). After the transfer, NOD-FMT mice experienced a dramatic change in the gut microbiomes and showed clinicopathological features of SS, including increased corneal fluorescein staining score, decreased tear production, elevated levels of IL-6 mRNA, decreased levels of MUC5AC mRNA encoding mucin. Additionally, we observed that NOD-FMT mice shared stereotypic B cell receptor (BCR) clonotypes with a much higher frequency compared to control group. B cell clones encoding these stereotypic BCR clonotypes developed and expanded locally in the lacrimal gland, and achieved systemic presence in certain clonotypes.
|
| |
|
| Overall design |
Feces were collected from 30 NOD.B10.H2b mice older than 20 weeks with autoimmune dry eye disease, and their microbiomes were transferred to ten mice (NOD-FMT group). In parallel, ten mice were orally gavaged with PBS (PBS-T group), and five mice from the same cohort received no treatment (NC group).
|
| |
|
| Contributor(s) |
Lee S, Ju S, Kwon S, Chung J, Kim M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 09, 2024 |
| Last update date |
Jun 27, 2024 |
| Contact name |
Soobin Lee |
| E-mail(s) |
lsb1234561@gmail.com
|
| Organization name |
Seoul National University
|
| Street address |
1, Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea
|
| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (48)
|
| GSM8194761 |
C57BL/6, NC1, lacrimal gland, biological rep1 |
| GSM8194762 |
C57BL/6, NC1, spleen, biological rep1 |
| GSM8194763 |
C57BL/6, NC1, lacrimal gland, biological rep2 |
| GSM8194764 |
C57BL/6, NC1, spleen, biological rep2 |
| GSM8194765 |
C57BL/6, NC1, lacrimal gland, biological rep3 |
| GSM8194766 |
C57BL/6, NC1, spleen, biological rep3 |
| GSM8194767 |
C57BL/6, NC1, lacrimal gland, biological rep4 |
| GSM8194768 |
C57BL/6, NC1, spleen, biological rep4 |
| GSM8194769 |
C57BL/6, NC1, lacrimal gland, biological rep5 |
| GSM8194770 |
C57BL/6, NC1, spleen, biological rep5 |
| GSM8194781 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep1 |
| GSM8194782 |
C57BL/6, NOD-FMT, spleen, biological rep1 |
| GSM8194783 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep10 |
| GSM8194784 |
C57BL/6, NOD-FMT, spleen, biological rep10 |
| GSM8194785 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep2 |
| GSM8194786 |
C57BL/6, NOD-FMT, spleen, biological rep2 |
| GSM8194787 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep3 |
| GSM8194788 |
C57BL/6, NOD-FMT, spleen, biological rep3 |
| GSM8194789 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep4 |
| GSM8194790 |
C57BL/6, NOD-FMT, spleen, biological rep4 |
| GSM8194791 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep5 |
| GSM8194792 |
C57BL/6, NOD-FMT, spleen, biological rep5 |
| GSM8194793 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep6 |
| GSM8194794 |
C57BL/6, NOD-FMT, spleen, biological rep6 |
| GSM8194795 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep7 |
| GSM8194796 |
C57BL/6, NOD-FMT, spleen, biological rep7 |
| GSM8194797 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep8 |
| GSM8194798 |
C57BL/6, NOD-FMT, spleen, biological rep8 |
| GSM8194799 |
C57BL/6, NOD-FMT, lacrimal gland, biological rep9 |
| GSM8194800 |
C57BL/6, NOD-FMT, spleen, biological rep9 |
| GSM8194801 |
C57BL/6, PBS-T, lacrimal gland, biological rep1 |
| GSM8194802 |
C57BL/6, PBS-T, spleen, biological rep1 |
| GSM8194803 |
C57BL/6, PBS-T, lacrimal gland, biological rep2 |
| GSM8194804 |
C57BL/6, PBS-T, spleen, biological rep2 |
| GSM8194805 |
C57BL/6, PBS-T, lacrimal gland, biological rep3 |
| GSM8194806 |
C57BL/6, PBS-T, spleen, biological rep3 |
| GSM8194807 |
C57BL/6, PBS-T, lacrimal gland, biological rep4 |
| GSM8194808 |
C57BL/6, PBS-T, spleen, biological rep4 |
| GSM8194809 |
C57BL/6, PBS-T, lacrimal gland, biological rep5 |
| GSM8194810 |
C57BL/6, PBS-T, spleen, biological rep5 |
| GSM8194811 |
C57BL/6, PBS-T, lacrimal gland, biological rep6 |
| GSM8194812 |
C57BL/6, PBS-T, spleen, biological rep6 |
| GSM8194813 |
C57BL/6, PBS-T, lacrimal gland, biological rep7 |
| GSM8194814 |
C57BL/6, PBS-T, spleen, biological rep7 |
| GSM8194815 |
C57BL/6, PBS-T, lacrimal gland, biological rep8 |
| GSM8194816 |
C57BL/6, PBS-T, spleen, biological rep8 |
| GSM8194817 |
C57BL/6, PBS-T, lacrimal gland, biological rep9 |
| GSM8194818 |
C57BL/6, PBS-T, spleen, biological rep9 |
|
| Relations |
| BioProject |
PRJNA1098088 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE263577_RAW.tar |
667.8 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...