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| Status |
Public on Jun 02, 2011 |
| Title |
Expression data from human amnion mesenchymal cells treated with interleukin-1-beta for 1hr and 8hr |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Premature birth continues to be a challenging pregnancy complication, and a body of literature indicates that inflammation can contribute to premature delivery by converting a receptive uterine environment to a hostile one. Cytokines have been demonstrated to provoke up-regulation of inflammatory genes (e.g. interleukin-1, 6, and 8, tumor necrosis factor-alpha, cyclooxygenase-2, and microsomal prostaglandin E synthase-1).
Using monolayer cultures of human amnion mesenchymal cells (AMCs) as a model, we aimed to detail the global programme of gene expression that occurs in response to cytokine challenge.
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| Overall design |
Human amnion mesenchymal cells were challenged with 10 ng/ml of interleukin-1-beta for 1 hour (1h) or 8 hours (8h) in serum-free media, while control cells were treated with serum free medium only (veh). The cells (veh, 1h and 8h) were subjected to RNA extraction, quantification, and evaluation, followed by hybridization on Affymetrix GeneChip® Human Genome U133A 2.0 Arrays (Platform GPL571). Experiments were repeated three times for a total of nine samples used for microarray analysis. Three biological replicates were used to ensure that any results were not biased by day-to-day variation in RNA extraction.
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| Contributor(s) |
Li R, Ackerman IV WE, Summerfield TL, Yu L, Galati P, Zhang J, Huang K, Romero R, Kniss DA |
| Citation(s) |
21655103 |
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| Submission date |
Dec 27, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Douglas A. Kniss |
| Organization name |
The Ohio State University
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| Department |
OB/GYN
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| Lab |
Laboratory of Perinatal Research
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| Street address |
395 W. 12th Avenue
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (9)
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| GSM646173 |
amnion mesenchymal cells, no treatment, biological rep1 |
| GSM646174 |
amnion mesenchymal cells, no treatment, biological rep2 |
| GSM646175 |
amnion mesenchymal cells, no treatment, biological rep3 |
| GSM646176 |
amnion mesenchymal cells, 1h interleukin-1-beta, biological rep1 |
| GSM646177 |
amnion mesenchymal cells, 1h interleukin-1-beta, biological rep2 |
| GSM646178 |
amnion mesenchymal cells, 1h interleukin-1-beta, biological rep3 |
| GSM646179 |
amnion mesenchymal cells, 8h interleukin-1-beta, biological rep1 |
| GSM646180 |
amnion mesenchymal cells, 8h interleukin-1-beta, biological rep2 |
| GSM646181 |
amnion mesenchymal cells, 8h interleukin-1-beta, biological rep3 |
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| Relations |
| BioProject |
PRJNA136761 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26315_RAW.tar |
17.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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