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| Status |
Public on Apr 11, 2024 |
| Title |
A body-brain circuit regulating body inflammatory responses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The body-brain axis is emerging as a principal conductor of organismal physiology. It senses and controls organ function, metabolism and nutritional state. Here, we show that a peripheral immune insult powerfully activates the body-brain axis to regulate immune responses. We demonstrate that pro- and anti-inflammatory cytokines communicate with distinct populations of vagal neurons to inform the brain of an emerging inflammatory response. In turn, the brain tightly modulates the course of the peripheral immune response. Genetic silencing of this body-to-brain circuit produced unregulated and out-of-control inflammatory responses. By contrast, activating, rather than silencing, this circuit affords exceptional neural control of immune responses. We used single-cell RNA sequencing, combined with functional imaging, to identify the circuit components of this neuro-immune axis, and showed that its selective manipulation can effectively suppress the pro-inflammatory response while enhancing an anti-inflammatory state. The brain-evoked transformation of the course of an immune response offers new possibilities in the modulation of a wide range of immune disorders, from autoimmune diseases to cytokine storm and shock.
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| Overall design |
cells from the nucleus of the solitary tract were isolated by enzymatic digestion and fluorescence-activated cell sorting based on the presence or absence of tdTom reporter expression and analyzed using scRNA-seq
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| Contributor(s) |
Jin H, Goltseker K, Zuker C |
| Citation missing |
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| Submission date |
Apr 02, 2024 |
| Last update date |
Apr 12, 2024 |
| Contact name |
Hao Jin |
| Organization name |
Columbia University
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| Street address |
3227 Broadway
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10027 |
| Country |
USA |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1095469 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE263025_RAW.tar |
1.0 Gb |
(http)(custom) |
TAR (of H5, TAR, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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