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Status |
Public on May 30, 2011 |
Title |
Pin1/mutant-p53 jointly controlled genes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To investigate the specific gene expression program by which mutant-p53 and Pin1 control invasion and metastasis in breast cancer cells, we compared the transcriptomic profile of control, mutant-p53 depleted or Pin1 depleted MDA-MB-231 cells.
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Overall design |
MDA-MB-231 cells were transfected twice with siRNA against Pin1, p53 or LacZ as a control. Transfections were performed by using Lifofectamine 2000 (Invitrogen) according to manufacture's procedure. Forty-eight hours after second transfection, samples were then processed for total RNA extraction and hybridization on Affymetrix microarrays. Three biological replicas (A, B, C) were used for each of the three conditions, for a total of 9 samples
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Contributor(s) |
Girardini JE, Napoli M, Piazza S, Rustighi A, Marotta C, Radaelli E, Capaci V, Jordan L, Quinlan P, Thompson A, Mano M, Rosato A, Crook T, Scanziani E, Means AR, Lozano G, Schneider C, DelSal G |
Citation(s) |
21741598 |
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Submission date |
Dec 22, 2010 |
Last update date |
Dec 06, 2018 |
Contact name |
Silvano Piazza |
E-mail(s) |
silvano.piazza@icgeb.org
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Organization name |
ICGEB
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Lab |
Computational Biology
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Street address |
localita' Padriciano 99
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City |
Trieste |
ZIP/Postal code |
34149 |
Country |
Italy |
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Platforms (1) |
GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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Samples (9)
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Relations |
BioProject |
PRJNA135101 |
Supplementary file |
Size |
Download |
File type/resource |
GSE26262_RAW.tar |
19.8 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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