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| Status |
Public on Mar 20, 2024 |
| Title |
The functional role of L-fucose on dendritic cell function and polarization |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Despite significant advances in the development and refinement of immunotherapies administered to combat cancer over the past decades, a number of barriers continue to limit their efficacy. One significant clinical barrier is the inability to mount initial immune responses towards the tumor. As dendritic cells are central initiators of immune responses in the body, the elucidation of mechanisms that can be therapeutically leveraged to enhance their functions to drive anti-tumor immune responses is urgently needed. Here, we report that the dietary sugar L-fucose can be used to enhance the immunostimulatory activity of dendritic cells (DCs). L-fucose polarizes immature myeloid cells towards specific DC subsets, specifically cDC1 and moDC subsets. In vitro, L-fucose treatment enhances antigen uptake and processing of DCs. Furthermore, our data suggests that L-fucose-treated DCs increase stimulation of T cell populations. Consistent with our functional assays, single-cell RNA sequencing of intratumoral DCs from tumor-bearing mice confirmed transcriptional regulation and antigen processing as pathways that are significantly altered by dietary L-fucose. Together, this study provides the first evidence of the ability of L-fucose to bolster DC functionality and provides rational to further investigate how L-fucose can be used to leverage DC function in order to enhance current immunotherapy.
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| Overall design |
To investigate the effect of L-fucose on immune subsets we took intratumoral CD45+ cells treated +\- L-fucose. We then preformed gene expression profiling comparing L-fucose-treated tumors to control-treated tumors at three time points. Comparative gene expression profiling analysis of RNA-seq data for intratumoral CD45+ cells with L-fucose-treated groups compared to control-treated groups.
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| Citation(s) |
38646527 |
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| Submission date |
Mar 19, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Dongliang Du |
| E-mail(s) |
dongliang.du@moffitt.org
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| Organization name |
Moffitt Cancer Center
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| Street address |
12902 Magnolia Dr
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| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| GSM8155368 |
CD45+ sorted tumor, control treated, day 0 RNA |
| GSM8155369 |
CD45+ sorted tumor, control treated, day 21 RNA |
| GSM8155370 |
CD45+ sorted tumor, control treated, day 7 RNA |
| GSM8155371 |
CD45+ sorted tumor, L-fucose treated, day 21 RNA |
| GSM8155372 |
CD45+ sorted tumor, L-fucose treated, day 7 RNA |
| GSM8155373 |
CD45+ sorted tumor, control treated, day 0 VDJ |
| GSM8155374 |
CD45+ sorted tumor, control treated, day 21 VDJ |
| GSM8155375 |
CD45+ sorted tumor, control treated, day 7 VDJ |
| GSM8155376 |
CD45+ sorted tumor, L-fucose treated, day 21 VDJ |
| GSM8155377 |
CD45+ sorted tumor, L-fucose treated, day 7 VDJ |
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| Relations |
| BioProject |
PRJNA1089764 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE261971_RAW.tar |
275.1 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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