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Series GSE261732 Query DataSets for GSE261732
Status Public on Dec 05, 2024
Title Dysregulation of microbiota gut-retina axis in neovascular age-related macular degeneration: beneficial ocular effects of a lutein, zeaxanthin and saffron based prebiotic supplementation
Organism human feces metagenome
Experiment type Other
Summary This work investigates the effects of a prebiotic mix containing lutein, zeaxanthin, and saffron, recognized for their anti-inflammatory properties, on ophthalmological and microbial parameters in neovascular AMD (nAMD) patients.
 
Overall design Total DNA was extracted from the stool of healthy old subjects and ADM (Age-related macular degeneration) patients. Moreover, the total DNA was extracted from stool samples of the same ADM patients after the completion of the prebiotic treatment. The total DNA was amplified to collect amplicons of variable V3–V4 regions (primers 341F and 805R) of the bacterial 16s rRNA gene and sequenced with MiSeq (2x300bp) Illumina platform.
 
Contributor(s) Ramazzotti M, Amedei A, Di Gloria L, Baldi S
Citation(s) 39599758
Submission date Mar 15, 2024
Last update date Dec 05, 2024
Contact name Matteo Ramazzotti
E-mail(s) matteo.ramazzotti@unifi.it
Organization name University of Florence
Department Experimental and Clinical Biomedical Sciences
Street address viale Morgagni 50
City Firenze
ZIP/Postal code 50134
Country Italy
 
Platforms (1)
GPL29470 Illumina MiSeq (human feces metagenome)
Samples (60)
GSM8150452 Reads obtained from sequencing of sample EN12
GSM8150453 Reads obtained from sequencing of sample EN13
GSM8150454 Reads obtained from sequencing of sample EN17
Relations
BioProject PRJNA1088508

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE261732_dna-sequences.fasta.gz 243.6 Kb (ftp)(http) FASTA
GSE261732_feature_table.tsv.gz 105.0 Kb (ftp)(http) TSV
GSE261732_taxonomy.tsv.gz 70.6 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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