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Series GSE26169 Query DataSets for GSE26169
Status Public on Dec 17, 2013
Title Expression data for Saccharomyces cerevisiae oxidative stress response
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response to oxidative stress.
We used microarrays to study the genome-wide temporal response of the yeast S. cerevisiae to oxidative stress induced by cumene hydroperoxide. Keywords: time course
Overall design The effects of oxidative stress induced by CHP on the transcriptional profile of S. cerevisiae was studied from a dynamical perspective. Yeast cultures were grown in controlled batch conditions, in 1 L fermentors. Three replicate cultures in mid-exponential phase were exposed to 0.19 mM CHP, while three non-treated cultures were used as controls. Samples were collected at t=0 (immediately before adding CHP) and at 3, 6, 12 and 20 min after adding the oxidant. Samples were processed for RNA extraction and profiled using Affymetrix Yeast Genome S98 arrays.
Contributor(s) Camacho D, Laubenbacher R, Martins A, Mendes P, Sha W, Shulaev V, Stigler B
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Submission date Dec 18, 2010
Last update date Jul 01, 2016
Contact name Pedro Mendes
Organization name University of Manchester
Department Manchester Institute of Biotechnology
Street address 131 Princess Street
City Manchester
ZIP/Postal code M1 7DN
Country United Kingdom
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (210)
GSM642444 Glr1 mutant, Control, t=0 min, biological replicate 1
GSM642445 Glr1 mutant, Control, t=3 min, biological replicate 1
GSM642446 Glr1 mutant, Control, t=6 min, biological replicate 1
BioProject PRJNA135247

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26169_RAW.tar 260.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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