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| Status |
Public on Dec 03, 2024 |
| Title |
Transcription factor 21 regulates cardiac myofibroblast formation and fibrosis [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcription factor 21 (TCF21) is a basic helix-loop-helix protein required for developmental specification of cardiac fibroblasts from epicardial progenitor cells that normally surround and invade the heart. In the adult heart, TCF21 is expressed in tissue-resident fibroblasts but is downregulated in response to injury or stimuli leading to myofibroblast differentiation. These findings led to the hypothesis that Tcf21 could be a regulator of fibroblast cell fate in the adult mammalian heart and contribute to cardiac fibrosis. Here, single-cell RNA-sequencing was used to study the effect of loss of Tcf21 in cardiac fibroblasts in adult mouse hearts at baseline and after an ischemic cardiac injury (myocardial infarction).
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| Overall design |
To investigate the function of TCF21 in vivo, TCF21 was conditionally deleted in cardiac fibroblasts. Mice were either studied in the absense of injury or after 3 days of myocardial infarction. Cardiac tissue was digested to obtain a single cell suspension and live nucleated cells were sorted for single cell RNA sequencing.
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| Contributor(s) |
Johansen AK, Molkentin JD |
| Citation(s) |
39629593 |
| |
| Submission date |
Mar 08, 2024 |
| Last update date |
Mar 04, 2025 |
| Contact name |
Jeffery D Molkentin |
| E-mail(s) |
jeff.molkentin@cchmc.org
|
| Organization name |
Cincinnati Children’s Hospital Medical Center
|
| Department |
Molecular Cardiovascular Biology
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| Lab |
Molkentin
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
|
| GSM8136447 |
Tcf21-MCM/fl myocardial infarction; scRNA-seq |
|
| Relations |
| BioProject |
PRJNA1085631 |
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