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Series GSE261150 Query DataSets for GSE261150
Status Public on Dec 03, 2024
Title Transcription factor 21 regulates cardiac myofibroblast formation and fibrosis [scRNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Transcription factor 21 (TCF21) is a basic helix-loop-helix protein required for developmental specification of cardiac fibroblasts from epicardial progenitor cells that normally surround and invade the heart. In the adult heart, TCF21 is expressed in tissue-resident fibroblasts but is downregulated in response to injury or stimuli leading to myofibroblast differentiation. These findings led to the hypothesis that Tcf21 could be a regulator of fibroblast cell fate in the adult mammalian heart and contribute to cardiac fibrosis. Here, single-cell RNA-sequencing was used to study the effect of loss of Tcf21 in cardiac fibroblasts in adult mouse hearts at baseline and after an ischemic cardiac injury (myocardial infarction).
 
Overall design To investigate the function of TCF21 in vivo, TCF21 was conditionally deleted in cardiac fibroblasts. Mice were either studied in the absense of injury or after 3 days of myocardial infarction. Cardiac tissue was digested to obtain a single cell suspension and live nucleated cells were sorted for single cell RNA sequencing.
 
Contributor(s) Johansen AK, Molkentin JD
Citation(s) 39629593
Submission date Mar 08, 2024
Last update date Mar 04, 2025
Contact name Jeffery D Molkentin
E-mail(s) jeff.molkentin@cchmc.org
Organization name Cincinnati Children’s Hospital Medical Center
Department Molecular Cardiovascular Biology
Lab Molkentin
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM8136444 Tcf21-MCM baseline; scRNA-seq
GSM8136445 Tcf21-MCM/fl baseline; scRNA-seq
GSM8136446 Tcf21-MCM myocardial infarction; scRNA-seq
Relations
BioProject PRJNA1085631

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Supplementary file Size Download File type/resource
GSE261150_RAW.tar 374.8 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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