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Series GSE26097 Query DataSets for GSE26097
Status Public on Jan 25, 2011
Title Pluripotency Factors Regulate Definitive Endoderm Specification through Eomesodermin
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Understanding the molecular mechanisms controlling early cell fate decisions in mammals is a major objective towards the development of robust methods for the differentiation of human pluripotent stem cells into clinically relevant cell types. Here, we used human embryonic stem cells (hESCs) to study specification of definitive endoderm in vitro. Using a combination of whole genome expression and ChIP-seq analyses, we established a hierarchy of transcription factors regulating endoderm specification. Importantly, pluripotency factors, namely NANOG, OCT4 and SOX2 have an essential function in this network by actively directing differentiation. Indeed, these transcription factors control the expression of EOMES, which marks the onset of endoderm specification. In turn, EOMES interacts with SMAD2/3 to initiate the transcriptional network governing endoderm formation. Together, these results provide for the first time a comprehensive molecular model connecting the transition from pluripotency to endoderm specification during mammalian development.
Overall design ChIP-Seq of Eomesodermin binding in human embyonic stem cells, differentiated towards an endodermal fate for 48h in chemically-defined culture media. Includes an input DNA control.

Supplementary file GSE26097_README.txt contains descriptions of the raw data files and processed data files.
Contributor(s) Teo AK, Trotter M
Citation(s) 21245162
Submission date Dec 16, 2010
Last update date May 15, 2019
Contact name Matthew Trotter
Organization name University of Cambridge
Department Department of Surgery
Lab Laboratory for Regenerative Medicine
Street address West Forvie Building, Forvie Site, Robinson Way
City Cambridge
ZIP/Postal code CB2 0SZ
Country United Kingdom
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (2)
GSM640690 hESC_Input_XL_ChIP-Seq
GSM640691 hESC_48h-endodiff_EOMES_XL_ChIP-Seq_reps1and2
SRA SRP004885
BioProject PRJNA135173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26097_RAW.tar 2.0 Gb (http)(custom) TAR (of BED, GFF, TXT)
GSE26097_README.txt 891 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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