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Series GSE26083 Query DataSets for GSE26083
Status Public on Dec 20, 2010
Title Genome-wide maps of Tamoxifen resistance MCF7 cell line
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the ER alpha regulatory network of Tamoxifen resistance MCF7 cell line using the Chromatin immunoprecipitated high-throughput sequencing technology (ChIP-seq). By Integrating the gene expression data (previously reported) with the ChIP-seq data, we generated ER alpha regulatory network and pathways. For ER alpha regulatory network, hub TFs with enriched motifs were identified from ER alpha peak together with PolII peaks. We then scan the position weight matrix (PWM) of ER alpha peak region of certain gene to find out the regulatory relationship between hub TF and normal TF. For regulatory pathway, genes were grouped base on their expression value at 4 different time point. Then the hub TF that plays important role in each time point of each group was identified. This study provides a framework for the application of ChIP-seq and gene expression data for the construction of ER alpha regulatory network.
 
Overall design 4 different ChIP-seq dataset in Tamoxifen resistance MCF7 cell line
 
Contributor(s) Gu F, Hsu H, Hsu P, Wu J, Ma Y, Parvin J, Huang TH, Jin VX
Citation(s) 21167036
Submission date Dec 15, 2010
Last update date May 15, 2019
Contact name Fei Gu
E-mail(s) alickgf@hotmail.com
Organization name The Ohio State University
Department Molecular Virology, Immunology & Medical Genetics
Lab Human Cancer Genetics, 814, Biomedical Research Tower
Street address 460 W. 12th Ave.
City Columbus
State/province Ohio
ZIP/Postal code 43210
Country USA
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (4)
GSM640419 PolII control
GSM640420 PolII E2 treat
GSM640421 ER control
Relations
SRA SRP004916
BioProject PRJNA135221

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26083_RAW.tar 202.3 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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