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| Status |
Public on Apr 12, 2024 |
| Title |
Mitochondrial ACSS1-K-635 acetylation mimic knock-in mice exhibit altered lipid metabolism, hepatic cell senescence, and acute non-alcoholic fatty liver [2ndSet_analysis] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We generated an ACSS1-acetylation (Ac) mimic mouse, where lysine 635 was mutated to glutamine (K635Q). Male Acss1K635Q/K635Q mice were smaller with higher metabolic rate and blood acetate, and decreased liver/serum ATP and lactate levels. After a 48-hour fast, Acss1K635Q/K635Q mice presented hypothermia and liver aberrations, including enlargement, discoloration, lipid droplet accumulation, and microsteatosis – consistent with nonalcoholic fatty liver disease (NAFLD). RNAseq analysis suggested dysregulation of fatty acid metabolism, cellular senescence, and hepatic steatosis networks, consistent with NAFLD
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| Overall design |
Fasted and fed C57BL/6J WT and ACSS1K635Q mice were studied in the context of non-alcoholic fatty liver disease. The liver tissue was collected and prepared for RNAseq as well as for Western blotting and qPCR analysis.
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| Contributor(s) |
Gius D, Xu G, Schell J, Horikoshi N, Chen Y, Lai Z |
| Citation(s) |
38758779 |
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| Submission date |
Feb 29, 2024 |
| Last update date |
May 29, 2024 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
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| Phone |
2105629163
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| Organization name |
UT Health Science Center at San Antonio
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| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM8119705 |
liver, ACSS1K635Q, fasted, biol rep 1 |
| GSM8119706 |
liver, ACSS1K635Q, control diet, biol rep 1 |
| GSM8119707 |
liver, ACSS1K635Q, control diet, biol rep 2 |
| GSM8119708 |
liver, WT, fasted, biol rep 1 |
| GSM8119709 |
liver, WT, control diet, biol rep 1 |
| GSM8119710 |
liver, WT, control diet, biol rep 2 |
| GSM8119711 |
liver, WT, control diet, biol rep 3 |
| GSM8119712 |
liver, ACSS1K635Q, fasted, biol rep 2 |
| GSM8119713 |
liver, ACSS1K635Q, fasted, biol rep 3 |
| GSM8119714 |
liver, ACSS1K635Q, control diet, biol rep 3 |
| GSM8119715 |
liver, WT, fasted, biol rep 2 |
| GSM8119716 |
liver, WT, fasted, biol rep 3 |
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| Relations |
| BioProject |
PRJNA1082272 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE260582_RAW.tar |
5.0 Mb |
(http)(custom) |
TAR (of RESULTS) |
SRA Run Selector |
| Raw data are available in SRA |
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