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Series GSE25947 Query DataSets for GSE25947
Status Public on Dec 10, 2010
Title E16-20_H3K9Ac
Project modENCODE
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary modENCODE_submission_411
This submission comes from a modENCODE project of Kevin White. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: The White Lab is aiming to map the association of the main post-translationally modified histones on the genome of Drosophila melanogaster. One technique that we use for this purpose is chromatin immunoprecipitation-on-chip (ChIP-on-chip) utilizing whole-genome tiling arrays. The data generated by ChIP-chip experiments consist basically of a plot of signal intensity across the genome. The highest signals correspond to positions in the genome occupied by the tested histone modification.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
CHIP-chip
 
Overall design EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Y cn bw sp(genotype : y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1] official name : y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1] ); Developmental Stage: E16-20; Genotype: y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1]; Sex: Unknown; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Developmental Stage E16-20; Antibody H3K9Ac (target is H3K9Ac); Strain Y cn bw sp(genotype : y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1] official name : y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1] )
Web link http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
 
Contributor(s) White K, Negre N, Bild N
Citation missing Has this study been published? Please login to update or notify GEO.
BioProject PRJNA63467
Submission date Dec 09, 2010
Last update date Aug 19, 2013
Contact name DCC modENCODE
E-mail help@modencode.org
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Lab modENCODE DCC
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (3)
GPL6949 Agilent-019182 Drosophila melanogaster Whole Genome ChIP-on-Chip Set 244K, Microarray 1 of 3
GPL6950 Agilent-019183 Drosophila melanogaster Whole Genome ChIP-on-Chip Set 244K, Microarray 2 of 3
GPL6951 Agilent-019184 Drosophila melanogaster Whole Genome ChIP-on-Chip Set 244K, Microarray 3 of 3
Samples (9)
GSM637608 E16-20_H3K9Ac_1 extraction1_array1
GSM637609 E16-20_H3K9Ac_1 extraction1_array2
GSM637610 E16-20_H3K9Ac_1 extraction1_array3

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25947_E16-20_H3K9Ac_DCC_merged.wig.gz 8.0 Mb (ftp)(http) WIG
GSE25947_E16-20_H3K9Ac_merged_dcc.gff.gz 38.1 Kb (ftp)(http) GFF
GSE25947_RAW.tar 619.0 Mb (http)(custom) TAR (of TXT)
GSE25947_agilent_labeling_protocol.pdf.gz 76.3 Kb (ftp)(http) PDF
Raw data provided as supplementary file
Processed data is available on Series record

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