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| Status |
Public on Feb 15, 2025 |
| Title |
Single-cell profiling of the nucleus pulposus (NP) supports the Shh+ cells as NP stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The physiological function of the intervertebral disc (IVD) depends on the cellular and molecular composition of the nucleus pulposus (NP), which plays a key role in managing the mechanical loading of the IVD.We conducted single-cell RNA sequencing (scRNA-seq) analysis to better characterize the NP cells, particularly the Shh-tdTom+ NP cells, in the adult Shh-CreER; Rosa26tdTomato mice.
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| Overall design |
After five consecutive Tamoxifen injections to the 1-month-old Shh-CreER; Rosa26tdTomato mice, lumbar and coccygeal IVDs were harvested 2 weeks later. CD45+ immune cells were removed by Fluorescence-Activated Cell Sorting (FACS) to focus on the non-immune cells in the IVD. The digested IVD cells were then subjected to 10X Genomics scRNA-seq.
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| Contributor(s) |
Liang G, Jin Y, Cong Q, Hu Y, Liu Y, Yu T, Yang Y |
| Citation missing |
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| Submission date |
Feb 09, 2024 |
| Last update date |
Feb 15, 2025 |
| Contact name |
Yuchen Liu |
| E-mail(s) |
yuchen_liu@hsdm.harvard.edu
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| Organization name |
Harvard school of dental medicine
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| Lab |
Yingzi Yang Lab
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| Street address |
188 Longwood Avenue, REB 514B
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1075104 |
