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Series GSE25520 Query DataSets for GSE25520
Status Public on Nov 25, 2010
Title PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse
Organism Homo sapiens
Experiment type Protein profiling by protein array
Summary To comprehensively analyze the effects of mTORC1 inhibition on GSK3, we employed the use of a PI3K/mTOR-specific phospho-antibody microarray that analyzed the site-specific phosphorylation of over 130 kinases within the PI3K/mTOR pathway. The phosphorylation levels of different kinases in monocytes were measured when stimulated with LPS in the presence or absence of a kind of mTORC1 inhibitor, rapamycin
Overall design More than 130 highly specific and characterized phospho-antibodies for the human mTOR signaling pathway were immobilized and replicated six times on glass slides. The same non-phosphorylated target antibodies were included to allow the determination of the relative level of phosphorylatioin
Contributor(s) Martin M, Liang R, wang H
Citation(s) 21422248
Submission date Nov 22, 2010
Last update date Mar 22, 2012
Contact name huizhi wang
Phone 502 852-1589
Organization name University of Louisville
Department PEDH
Lab Dr. Martin
Street address 501 S.Preston St. Room 211B
City louisville
State/province kY
ZIP/Postal code 40202
Country USA
Platforms (1)
GPL11222 mTOR Signaling Phospho Specific Antibody Microarray
Samples (2)
GSM624618 LPS
GSM624619 LPS+Rapamycin
BioProject PRJNA133777

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25520_RAW.tar 70.0 Kb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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