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| Status |
Public on Nov 25, 2010 |
| Title |
PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse |
| Organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
|
| Summary |
To comprehensively analyze the effects of mTORC1 inhibition on GSK3, we employed the use of a PI3K/mTOR-specific phospho-antibody microarray that analyzed the site-specific phosphorylation of over 130 kinases within the PI3K/mTOR pathway. The phosphorylation levels of different kinases in monocytes were measured when stimulated with LPS in the presence or absence of a kind of mTORC1 inhibitor, rapamycin
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| Overall design |
More than 130 highly specific and characterized phospho-antibodies for the human mTOR signaling pathway were immobilized and replicated six times on glass slides. The same non-phosphorylated target antibodies were included to allow the determination of the relative level of phosphorylatioin
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| Contributor(s) |
Martin M, Liang R, wang H |
| Citation(s) |
21422248 |
| Submission date |
Nov 22, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
huizhi wang |
| E-mail(s) |
hhwang01@louisville.edu
|
| Phone |
502 852-1589
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| Organization name |
University of Louisville
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| Department |
PEDH
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| Lab |
Dr. Martin
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| Street address |
501 S.Preston St. Room 211B
|
| City |
louisville |
| State/province |
kY |
| ZIP/Postal code |
40202 |
| Country |
USA |
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| Platforms (1) |
| GPL11222 |
mTOR Signaling Phospho Specific Antibody Microarray |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA133777 |
