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| Status |
Public on Jul 25, 2024 |
| Title |
RNA-seq analysis of wild-type HEK293, HEK293 treated with DMSO, ML216 or NSC617145, wild-type mESCs and dhx9KO mESCs |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
G4s are built by stacked guanine tetrads connected via Hoogsteen hydrogen bonds and can be formed by intra- or inter-molecular folding of the tetramers. R-loops are three-stranded structures which contain a DNA-RNA hybrid and a displaced single-stranded DNA. Both G4s and R-loops are involved in key biological processes, including transcriptional regulation, replication, genomic instability, class switch recombination in B cells, DNA damage and repair, and telomere maintenance. To understand the transcriptional regulation of G4s and R-loops, we performed the RNA-seq analysis on HEK293 cells treated with the inhibitors of G4 resolving helicase ML216 or NSC617145 and mESCs in which the resolving helicase DHX9 of G4 and R-loop was knockout.
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| Overall design |
To investigate the effect of ML216 and NSC617145 in HEK293,we treated HEK293 with corresponding helicase inhibitors and performed RNA-seq. To invistegate the effect of Dhx9 in mESC,we established dhx9 knockout mESC using CRISPR/Cas9 and performed RNA-seq.
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| Web link |
https://doi.org/10.7554/eLife.99026.1
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| |
|
| Contributor(s) |
Xue Z |
| Citation(s) |
39392462 |
| |
| Submission date |
Jan 31, 2024 |
| Last update date |
Oct 24, 2024 |
| Contact name |
Zhihong Xue |
| E-mail(s) |
xuez85@outlook.com
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| Organization name |
Sichuan University
|
| Street address |
No. 20, 3rd section, South Renmin Road
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA1071643 |
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