|
| Status |
Public on Jul 20, 2005 |
| Title |
MtIFS1-expressing alfalfa |
| Organism |
Medicago truncatula |
| Experiment type |
Expression profiling by array
|
| Summary |
We used an M. truncatula 16K oligonucleotide array to profile gene expression in the leaves of transgenic alfalfa plants expressing Medicago truncatula isoflavone synthase 1 (MtIFS1). RNA purified from leaves of MtIFS1-expressing lines C22 and B20 and vector control line VC11 was used to generate Cy5-labeled cDNA. RNA purified from a second vector control line, VB2, was used to generate Cy3-labeled reference cDNA. Three independently propagated cuttings of each line were used and a total of nine hybridizations were performed. Our results indicated that MtIFS1-expression does not significantly alter global gene expression in the leaves.
Keywords = Medicago
Keywords = isoflavone synthase
Keywords = alfalfa
Keywords: other
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| Contributor(s) |
Deavours BE, Dixon RA |
| Citation(s) |
16006598 |
| |
| Submission date |
Apr 19, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Bettina Deavours |
| E-mail(s) |
bedeavours@noble.org
|
| Phone |
580-224-6600
|
| Organization name |
Samuel Roberts Noble Foundation
|
| Street address |
|
| City |
Ardmore |
| State/province |
OK |
| ZIP/Postal code |
73401 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1971 |
Medicago truncatula Genome Oligo Set |
|
| Samples (9)
|
| GSM48532 |
VC11 vector control line replicate 1 |
| GSM48538 |
VC11 vector control line replicate 2 |
| GSM48540 |
VC11 vector control line replicate 3 |
| GSM48542 |
C22 MtIFS1-expressing line replicate 1 |
| GSM48544 |
C22 MtIFS1-expressing line replicate 2 |
| GSM48545 |
C22 MtIFS1-expressing line replicate 3 |
| GSM48547 |
B20 MtIFS1-expressing line replicate 1 |
| GSM48548 |
B20 MtIFS1-expressing line replicate 2 |
| GSM48549 |
B20 MtIFS1-expressing line replicate 3 |
|
| Relations |
| BioProject |
PRJNA92027 |
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