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| Status |
Public on May 29, 2024 |
| Title |
Transcriptional profiling of CD11b+ microglia isolated from cerebellum and olfactory bulb-removed brains of SAMP8 and SAMR1 mice at 2, 5, and 9 months of age. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
SAMP8 mice serve as an accelerated aging model characterized by inflammation and neurological memory deficits, in contrast to their control SAMR1 counterparts. To investigate the role of microglia in aging, we conducted a comprehensive analysis of microglial heterogeneity using CITE-seq. CD11b positive cells were extracted from cerebellum and olfactory bulb-removed brains at key time points: 2 months, 5 months, and 9 months of age.
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| Overall design |
At 2, 5, and 9 months of age, brains of SAMP8 and SAMR1 mice were prepared by removing the cerebellum and olfactory bulbs. The remaining brain tissue was then physically and enzymatically dissociated in presence of Actinomycin D and Brefeldin A. Following dissociation, CITE-seq antibodies (ADTs and HTOs) and CD11b-PE/Cy5 staining were used. The DAPI-CD11b+ cells were subsequently isolated using fluorescence-activated cell sorting (FACS).
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| Contributor(s) |
Gruel R, Bijnens B, Van Den Daele J, Thys S, Verstraelen P, Roels J, Mancuso R, Vandamme N, De Vos WH |
| Citation(s) |
38403918 |
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| Submission date |
Jan 18, 2024 |
| Last update date |
May 29, 2024 |
| Contact name |
VIB Single Cell Core |
| E-mail(s) |
jana.roels@vib.be, niels.vandamme@vib.be, data@single-cell.be
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| Organization name |
VIB
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| Department |
VIB Single cell core
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| Street address |
Technologiepark-Zwijnaarde 71
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| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| GSM8023839 |
9-months SAMP8, GEX |
| GSM8023840 |
9-months SAMP8, ADT |
| GSM8023841 |
9-months SAMP8,HTO |
| GSM8023842 |
5-months SAMR1, GEX |
| GSM8023843 |
5-months SAMR1, ADT |
| GSM8023844 |
5-months SAMR1,HTO |
| GSM8023845 |
5-months SAMP8, GEX |
| GSM8023846 |
5-months SAMP8, ADT |
| GSM8023847 |
5-months SAMP8,HTO |
| GSM8023848 |
2-months SAMR1, GEX |
| GSM8023849 |
2-months SAMR1, ADT |
| GSM8023850 |
2-months SAMR1,HTO |
| GSM8023851 |
2-months SAMP8, GEX |
| GSM8023852 |
2-months SAMP8, ADT |
| GSM8023853 |
2-months SAMP8,HTO |
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| Relations |
| BioProject |
PRJNA1066315 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE253582_RGR001_raw_feature_bc_matrix.h5 |
102.4 Mb |
(ftp)(http) |
H5 |
| GSE253582_RGR002_raw_feature_bc_matrix.h5 |
128.7 Mb |
(ftp)(http) |
H5 |
| GSE253582_RGR003_raw_feature_bc_matrix.h5 |
59.7 Mb |
(ftp)(http) |
H5 |
| GSE253582_RGR004_raw_feature_bc_matrix.h5 |
74.1 Mb |
(ftp)(http) |
H5 |
| GSE253582_RGR008_raw_feature_bc_matrix.h5 |
51.1 Mb |
(ftp)(http) |
H5 |
| GSE253582_RGR009_raw_feature_bc_matrix.h5 |
95.4 Mb |
(ftp)(http) |
H5 |
| GSE253582_SAMP8.rds.gz |
1.5 Gb |
(ftp)(http) |
RDS |
| GSE253582_readme.txt |
5.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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