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| Status |
Public on May 01, 2024 |
| Title |
A spatial transcriptomics based Label-free Method for Assessment of Human Stem Cell Distribution and Effects in a Mouse Model of Lung Fibrosis |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
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| Summary |
Recently, cell therapy has emerged as a promising treatment option for various disorders. Given the intricate mechanisms of action (MOA) and heterogenous distribution in target tissues inherent to cell therapy, it is necessary to develop more sophisticated, unbiased approaches to evaluate the distribution of administered cells and the molecular changes at a microscopic level. This study introduces a label-free approach for assessing the tissue distribution of administered human mesenchymal stem cells (hMSCs) and their MOA, leveraging spatially resolved transcriptomics (ST) analysis. The hMSCs were introduced into a mouse model with lung fibrosis, followed by the manipulation of ST to visualize the spatial distribution of hMSCs within the tissue. This was achieved by capitalizing on interspecies transcript differences between human and mouse. Furthermore, the method allowed for the examination of molecular changes associated with the spatial distribution of hMSCs. Therefore, our method has the potential to serve as an effective tool for various cell-based therapeutic agents.
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| Overall design |
We administered human mesenchymal stem cells (hMSC) in a mouse model of lung fibrosis and presented a method for evaluating both the tissue-level distribution of the administered stem cells and their effect on treated tissue by using Visium Spatial Transcriptomics.
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| Web link |
https://doi.org/10.1002/adtp.202300283
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| Contributor(s) |
Park J, Lee D, Lee JE, Lee D, Song IH, Park HS, Choi H, Im H |
| Citation |
J Park, D Lee, J E Lee, D Lee, I H Song, H S Park, H Choi, and H-J Im. A Spatial Transcriptomics Based Label-Free Method for Assessment of Human Stem Cell Distribution and Effects in a Mouse Model of Lung Fibrosis. Adv Therap 2024, 7, 2300283. doi:10.1002/adtp.202300283
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| Submission date |
Jan 16, 2024 |
| Last update date |
Aug 01, 2024 |
| Contact name |
Jeongbin Park |
| E-mail(s) |
jeongbin.park@portrai.io
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| Phone |
010-4071-6003
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| Organization name |
Seoul National University
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| Department |
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea
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| Lab |
Translational Molecular Imaging and Theranostics Laboratory
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| Street address |
23, Sinhyeon-ro 38beon-gil, Siheung-si, Gyeonggi-do, 14957, Rep. of KOREA
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| City |
Seoul |
| State/province |
Not US or Canada |
| ZIP/Postal code |
B14230324 |
| Country |
South Korea |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL25431 |
Illumina HiSeq 4000 (Homo sapiens; Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA1065433 |
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