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Series GSE25322 Query DataSets for GSE25322
Status Public on Nov 28, 2011
Title MRLxSM eQTL in Liver by RMA on Ensembl transcripts
Organism Mus musculus
Experiment type Expression profiling by array
Summary A QTL analysis between inbred mouse strains MRL/MpJ and SM/J was performed to identify genetic loci influencing high-density lipoprotein (HDL) cholesterol and triglycerides (TG) at eight weeks of age in F2 mice fed a chow diet. In order to narrow down lists of candidate genes, expression levels from liver tissue were used to test for differential expression among parental and F1 strains and to scan for eQTL in F2 animals. We provide evidence for Mppe1 (Chr 18) as an HDL QTL candidate gene and Cyp2d26 (Chr 15) as a TG QTL candidate gene.
Overall design MRL/MpJ (MRL) mice were crossed with male SM/J (SM) mice; their progeny were intercrossed to produce 371 F2 animals. Of these, 282 F2 males and females were profiled with Affy Mouse Gene 1.0 ST arrays for an eQTL study. Three animals from each parental and the F1 strains from each sex, i.e. 18 in total, were also profiled for testing differential expression. A total of 300 samples are included in the dataset. The mice were weaned at 3 weeks on a chow diet. At 13 weeks of age, mice were single housed for 3 days and fasted for 4 hours prior to being sacrificed. Tissue collection was performed between noon and 1pm to avoid variations due to circadian rhythm.
Contributor(s) Leduc MS, Hageman RS, Verdugo RA, Tsaih S, Walsh K, Churchill GA, Paigen B
Citation(s) 21622629, 22498810
Submission date Nov 12, 2010
Last update date Jul 13, 2012
Contact name Ricardo Alejandro Verdugo
Organization name The Jackson Laboratory
Department Research
Lab Gary A. Churchill
Street address 600 Main Street
City Bar Harbor
State/province ME
ZIP/Postal code 04609
Country USA
Platforms (1)
GPL11204 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENST]
Samples (300)
GSM622868 F2-2207-M
GSM622869 F2-2203-F
GSM622870 F2-2040-F
BioProject PRJNA134845

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25322_RAW.tar 1.2 Gb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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