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| Status |
Public on Feb 07, 2025 |
| Title |
Influence of GPR35 deficiency on the ileal and macrophage transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mutations in GPR35 (G protein coupled receptor 35) are linked to ulcerative colitis and primary sclerosing cholangitis. How GPR35, which interacts with the Na/K-ATPase to determine ion transport, influences the transcriptome of macrophages and the ileum is unknown. Here compare transcript expression in bone marrow-derived macrophages and isolated ileum from mice deficient in GPR35 (knockout) with wild type mice by bulk RNA sequencing.
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| Overall design |
Ileal tissue from wild type (n=5) and GPR35-deficient mice (n=5) was isolated and RNA extracted. Bone marrow-derived macrophages were cultured from wild type (n=5) and GPR35-deficient (n-5) mice and RNA extracted as described previously.
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| Contributor(s) |
Henriksen EK, Karlsen TH, Elias JE, Sewell GW, Kaneider NC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 10, 2024 |
| Last update date |
Feb 07, 2025 |
| Contact name |
Gavin Sewell |
| Organization name |
Cambridge Institute of Therapeutic Immunology and Infectious Disease
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| Department |
Jeffrey Cheah Biomedical Centre, University of Cambridge
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| Street address |
Puddicombe Way
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| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| GSM8010409 |
Ileum, wild type 4 |
| GSM8010410 |
Ileum, wild type 5 |
| GSM8010411 |
Ileum, GPR35 knockout 1 |
| GSM8010412 |
Ileum, GPR35 knockout 2 |
| GSM8010413 |
Ileum, GPR35 knockout 3 |
| GSM8010414 |
Ileum, GPR35 knockout 4 |
| GSM8010415 |
Ileum, GPR35 knockout 5 |
| GSM8010416 |
macrophage, wild type 1 |
| GSM8010417 |
macrophage, wild type 2 |
| GSM8010418 |
macrophage, wild type 3 |
| GSM8010419 |
macrophage, wild type 4 |
| GSM8010420 |
macrophage, wild type 5 |
| GSM8010421 |
macrophage, GPR35 knockout 1 |
| GSM8010422 |
macrophage, GPR35 knockout 2 |
| GSM8010423 |
macrophage, GPR35 knockout 3 |
| GSM8010424 |
macrophage, GPR35 knockout 4 |
| GSM8010425 |
macrophage, GPR35 knockout 5 |
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| Relations |
| BioProject |
PRJNA1063257 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE252926_RNAseq_GPR35_ileum.txt.gz |
741.1 Kb |
(ftp)(http) |
TXT |
| GSE252926_RNAseq_GPR35_macrophages.txt.gz |
696.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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