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| Status |
Public on Jan 02, 2024 |
| Title |
RNA sequencing of low grade neuroepithelial tumors associated with epilepsy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Low grade neuroepithelial tumor is the major cause of epilepsy Low-grade neuroepithelial tumors are major causes of drug-resistant focal epilepsy. The BRAF V600E mutation is frequently observed in low grade neuroepithelial tumor and linked to poor seizure outcomes. However, its molecular role in epileptogenicity remains elusive. To understand the molecular mechanism underlying the epileptogenicity in LEAT with the BRAF V600E genetic mutation (BRAF V600E-LEAT), we conducted RNA sequencing (RNA-seq) analysis using surgical specimens of BRAF V600E-LEAT obtained and stored at a single institute. bioinformatics analysis using this dataset identified 2,134 differentially expressed genes between BRAF V600E-LEAT and control. Additionally, gene set enrichment analysis provided novel insights into the association between estrogen response-related pathways and the epileptogenicity of BRAF V600E-LEAT patients.
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| Overall design |
We performed bulk RNAseq analysis using surgical specimens of 21 BRAF V600E-LEAT and 4 controls. All specimens were taken and stored in a single institute.
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| Contributor(s) |
Miyashita S, Iijima K, Taya S, Iwasaki M, Hoshino M |
| Citation(s) |
38269481 |
| BioProject |
PRJDB17032 |
| |
| Submission date |
Jan 01, 2024 |
| Last update date |
Jan 29, 2024 |
| Contact name |
Satoshi Miyashita |
| E-mail(s) |
s.miyashita@ncnp.go.jp
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| Organization name |
National Center of Neurology and Psychiatry
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| Department |
Institute of Neuroscience
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| Lab |
Biochemistry and cellular biology
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| Street address |
4-1-1, Ogawahigashimachi, Kodaira
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| City |
Tokyo |
| ZIP/Postal code |
187-8502 |
| Country |
Japan |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (25)
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