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| Status |
Public on Jan 25, 2024 |
| Title |
mRNA-based precision targeting of neoantigens and tumor-associated antigens in malignant brain tumors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
mRNA-based immunotherapeutics have garnered widespread interest for their potential to revolutionize cancer treatment. In this study, we have established a personalized and customizable mRNA-therapeutic approach that effectively targets a plurality of tumor antigens and demonstrated potent anti-tumor response in preclinical brain tumor models. Our platform uniquely addresses the challenge of tumor heterogeneity and low antigen burden, two key deficiencies in targeting classically immunotherapy-resistant CNS malignancies.
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| Overall design |
We created GBM tumor antigen-specific RNA and loaded it into dendritic cells for anti-tumor vaccine. We performed in vivo anti-tumor efficacy study with antigen loaded dendritic cells in combination with adoptive T cell therapy with relevant controls in the KR158 murine GBM model. Post therapy, we isolated the CD45+ve immune cells and CD45 -ve tumor cells from the brain tumor microenvironment and performed scRNA-seq analysis. The samples from each treatment arm were multiplexed together to minimize batch effect. Each treatment arm had 4 replicates for CD45 +ve immune cell subset divided across 3 multiplexed pools and 1 combined replicate for CD45 -ve tumor cells multiplexed into 1 pool.
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| Contributor(s) |
Trivedi V, Yang C, Micthell D |
| Citation missing |
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| BioProject |
PRJNA1054323 |
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| Submission date |
Dec 29, 2023 |
| Last update date |
Jan 25, 2024 |
| Contact name |
Duane Mitchell |
| E-mail(s) |
Duane.Mitchell@neurosurgery.ufl.edu
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| Organization name |
University of Florida
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| Street address |
1333 Center Drive, BSB B1-150
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| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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