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Series GSE25144 Query DataSets for GSE25144
Status Public on Nov 04, 2010
Title Comparison of effects of Iodine-deficiency and perchlorate on thyroid
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Perchlorate, which is a ubiquitous and persistent ion, competitively interferes with iodide accumulation in the thyroid, causing iodine deficiency, which may result in reduced thyroid hormone synthesis and secretion. Human studies suggest that perchlorate presents very little risk in healthy individuals; however, the precautionary principle demands that the sensitive populations of iodine deficient adults and mothers require extra consideration. In an attempt to determine if the effects on gene expression were similar, we compared the thyroidal effects of perchlorate (10 mg/kg) treatment for 14 days in drinking water with those caused by 8 weeks of Iodine-deficiency in rats. The thyroids were collected (N=3 each group) and total mRNA was analyzed using the Affymetrix Rat Genome 230 2.0 GeneChip®. Changes in gene expression were compared with appropriate control groups. We compared the 2-fold gene changes due to I-deficiency with changes due to perchlorate treatment. 189 transcripts were changed by the Iodine-deficient diet and 722 transcripts were changed by the perchlorate treatment. 34% of the transcripts changed by the I-deficient diet were also changed by perchlorate and generally in the same direction. three specific transporter genes, AQP1, NIS, & SLC22A3 were changed by both treatments, indicating that the membrane specific changes were similar. Iodine-deficiency primarily caused changes in retinol and calcium signaling pathways and perchlorate primarily caused changes related to the accumulation of extracellular matrix proteins. This study provides evidence that perchlorate, at least at this dose level, changes more genes and changes different genes compared to iodine deficiency.
 
Overall design Changes in gene expression due to I-deficiency compared to normal diet for 2 months.
Changes in gene expression due to perchlorate in the drinking water compared to normal drinking water for 1 or 14 days.
Feeding study in rats.
 
Contributor(s) Jones K, McDougal J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 04, 2010
Last update date Jul 31, 2017
Contact name Ken Jones
E-mail kenjones@uga.edu
Organization name University of Georgia
Department Environmental Health Science
Street address 150 E. Green St.
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (24)
GSM617969 iodine-deficient diet and perchlorate water A2
GSM617970 iodine-deficient diet and perchlorate water A3
GSM617971 iodine-deficient diet and perchlorate water A6
Relations
BioProject PRJNA134485

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25144_RAW.tar 72.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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