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| Status |
Public on Mar 27, 2024 |
| Title |
A single cell survey of CD127+ immune cells and EpCAM+ epithelial cells from wild type mice and Gabbr1-deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the role of GABAergic signaling in regulation of ILC3 function and intestinal tissue homeostasis, we performed single cell RNA sequencing to analyze intestinal CD127+ immune cells and EpCAM+ epithelial cells
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| Overall design |
Gabbr1flox/flox;Rorc-Cre- (Gabbr1WT) and Gabbr1flox/flox;Rorc-Cre+ (Gabbr1ΔRorc) mice were treated with 2.5% DSS for colitis induction. Small intestine tissues were dissociated with GEXSCOPE® Tissue Dissociation according to manufacturer's instructions. CD45+CD127+ immune cells and CD45-EpCAM+ epithelial cells were sorted using FACS, mixed at 1:1 ratio and subjected to scRNA-seq.
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| Contributor(s) |
Liu N, Liu B, Fan Z |
| Citation(s) |
40033120 |
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| Submission date |
Dec 18, 2023 |
| Last update date |
Mar 18, 2025 |
| Contact name |
Nian Liu |
| E-mail(s) |
liunian181@mails.ucas.ac.cn
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| Phone |
+8618100502042
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| Organization name |
Institute of Biophysics, Chinese Academy of Sciences
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| Street address |
Institute of Biophysics,#15 Datun Road
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| City |
Chaoyang Distric |
| State/province |
Beijing |
| ZIP/Postal code |
100010 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1054090 |
