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| Status |
Public on May 01, 2024 |
| Title |
Single cell gene and protein expression of 2W:I-Ab specific cells from effector and memory T cells after aLm infection. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single-cell gene expression of CD4+ tetramer+ cells from WT B6 animals was determined by sRNAseq after aLm infection.
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| Overall design |
B6 mice were infected with aLm for 6, 10, 15, 19, 22, 25, 30 or 60 days, treated wth an anti-ARTC2 nanobody before harvest, and sorted for CD4+ Tetramer+ cells. Post sort cells were hashtagged for each timepoint and feature barcoded for cell surface proteins and immune receptor mapping. Gene expression was determined by scRNAseq.
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| Contributor(s) |
Jenkins MK, Osum KC, Magill I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 14, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
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| Phone |
617-432-7747
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| Organization name |
Harvard Medical School
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| Department |
Microbiology and Immunobiology
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| Lab |
CBDM
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1052716 |
