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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 17, 2024 |
| Title |
CCDC88B interacts with RASAL3 and ARHGEF2 to regulate dendritic cell function in neuroinflammation and colitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CCDC88B is a risk factor for several chronic inflammatory diseases in humans and its inactivation causes a migratory defect in DCs in mice. CCDC88B belongs to a family of cytoskeleton-associate scaffold proteins that feature protein:protein interaction domains. Here, we identified the Rho/Rac Guanine Nucleotide Exchange Factor 2 (ARHGEF2) and the RAS Protein Activator Like 3 (RASAL3) as CCDC88B physical and functional interactors. Mice defective in Arhgef2 or Rasal3 show dampene neuroinflammation, and display altered cellular response and susceptibility to colitis; ARHGEF2 maps t a human Chromosome 1 locus associated with susceptibility to IBD. Arhgef2 and Rasal3 mutant DCs show altered migration and motility in vitro, causing either reduced (Arhgef2) or enhanced (Rasal3) migratory properties. The CCDC88B/RASAL3/ARHGEF2 complex appears to regulate DCs migration by modulating activation of RHOA, with ARHGEF2 and RASAL3 acting in opposite regulatory fashions, providing a molecular mechanism for the involvement of these proteins in DCs immune functions.
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| Overall design |
Single cell RNA sequencing of immune cells (CD45+) cell sorted from dissociated C57BL/6 mouse colons.
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| Contributor(s) |
Gros P, Fodil N, Jeyakumar T, Langlais D |
| Citation(s) |
38200184 |
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| Submission date |
Dec 05, 2023 |
| Last update date |
Jan 18, 2024 |
| Contact name |
David Langlais |
| E-mail(s) |
david.langlais@mcgill.ca
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| Organization name |
McGill University
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| Department |
Human Genetics
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| Lab |
Inflammation Genomics Lab
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| Street address |
740 Ave Dr Penfield, rm 4203, McGill Genome Centre
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| City |
MONTREAL |
| State/province |
QC |
| ZIP/Postal code |
H3A0G1 |
| Country |
Canada |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1048821 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE249342_RAW.tar |
106.7 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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