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Series GSE24886 Query DataSets for GSE24886
Status Public on Jan 07, 2011
Title Exosomes mediate protective messages during oxidative stress; role of exosomal shuttle RNA
Organism Mus musculus
Experiment type Expression profiling by array
Summary Exosomes are small extracellular nano-vesicles of endocytic origin that mediate different signals between cells, by surface interactions and by shuttling of functional RNA from one cell to another. In this study, we show that exosomes, produced by mouse mast cells exposed to oxidative stress, change their mRNA content and also that these exosomes can influence the response of other cells to oxidative stress by providing recipient cells with a resistance against oxidative stress. Finally, we also show that UV-light affect the biological functions associated with exosomes released under oxidative stress. These results argue that exosomal shuttle of RNA is involved in cell-to-cell communication, by influencing the response of recipient cells to an external stimulus.
 
Overall design We used microarrays to detail to examine the gene expression underlying the effect of H2O2 on the exosomal RNA and how exosomes isolated from oxidative stress influence the growth of recipient cells during oxidative stress. The mRNA content of the exosomes from normal conditions (MC9 Exo_norm) were compared to that from exosomes from H2O2 conditions (MC9 Exo_H2O2). The mRNA content of recipient cells MC9 were identified before (MC9 cells_norm) and after (MC9 cells_H2O2) addition of exosomes that were isolated from normal and H2O2 conditions.
 
Contributor(s) Eldh M, Ekström K, Valadi H, Sjöstrand M, Olsson B, Jernås M, Lötvall J
Citation(s) 21179422
Submission date Oct 22, 2010
Last update date Feb 11, 2019
Contact name Hadi Valadi
E-mail(s) hadi.valadi@gu.se
Phone +46(0)704561604
Organization name University of Gothenburg
Department Dept. of Rheumatology and Inflammation Research
Street address Guldhedsgatan 10A
City Gothenburg
ZIP/Postal code 405 30
Country Sweden
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM612027 Exosomes from normal conditions_rep 1
GSM612028 Exosomes from normal conditions_rep 2
GSM612029 Exosomes from normal conditions_rep 3
Relations
BioProject PRJNA132033

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24886_RAW.tar 55.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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