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| Status |
Public on Jan 24, 2024 |
| Title |
Effect of Regnase-2/MCPIP2/ZC3H12B overexpression in human glioblastoma U-251 MG cell line. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Regnase-2/MCPIP2/ZC3H12B is a protein belonging to the ZC3H12 protein family. There are three other proteins of this family present in humans of which Regnase-1/MCPIP1/ZC3H12A is the founder member as well as the best-described protein. All proteins of this family contain a NYN domain with endoribonuclease activity and a CCCH-type zinc finger responsible for interaction with substrate RNA. Although the NYN domain has a high degree of similarity across all four members the in vitro RNase activity was shown only for two of them, namely Regnase-1 and Regnase-3. Interestingly the ZC3H12 family proteins have unique expression patterns with Regnase-2 being expressed almost solely in the central nervous system. What is more, Regnase-2 is downregulated in human glioblastoma, and its expression is inversely correlated with tumor grade. To decipher the role of Regnase-2 in human glioblastoma we performed RNA sequencing of U-251 MG cells overexpressing Regnase-2 and control, mock-transfected cells.
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| Overall design |
To investigate the role of Regnase-2 in U-251 MG cells we established two U-251 MG cell lines with doxycycline-inducible overexpression of Regnase-2 or luciferase (control/mock) and we induced the expression of transgenes for 24 hours.
Differential expression profiling was performed using data obtained from RNA-seq of the two cell lines (outsourced to QIAGEN Genomic Services, Germany).
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| Contributor(s) |
Sowinska W, Wawro M, Kochan J, Kasza A |
| Citation(s) |
38238463 |
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| Submission date |
Nov 26, 2023 |
| Last update date |
Jan 24, 2024 |
| Contact name |
Mateusz Wawro |
| E-mail(s) |
mateusz.wawro@uj.edu.pl
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| Organization name |
Jagiellonian University
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| Department |
Cell Biochemistry
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| Lab |
Aneta Kasza
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| Street address |
Gronostajowa 7
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| City |
Kraków |
| ZIP/Postal code |
30-387 |
| Country |
Poland |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| GSM7918282 |
U-251 MG, Control, replicate 1 [20684-003] |
| GSM7918283 |
U-251 MG, Control, replicate 2 [20684-006] |
| GSM7918284 |
U-251 MG, Control, replicate 3 [20684-004] |
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| Relations |
| BioProject |
PRJNA1045288 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE248634_Expression_Browser.xlsx |
6.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
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