|
| Status |
Public on Apr 03, 2024 |
| Title |
Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [ampliseq 3] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions
|
| |
|
| Overall design |
Map the off-target landscape of 1 sgRNAs targeting VEGFAsite2 with Streptococcus pyogenes (Sp)Cas9 using TTISS-seq
|
| |
|
| Contributor(s) |
Longo GM, Sayols S, Roukos V |
| Citation(s) |
38740992 |
| |
| Submission date |
Nov 17, 2023 |
| Last update date |
Jul 03, 2024 |
| Contact name |
Sergi Sayols |
| Organization name |
Institute of Molecular Biology, Mainz
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (4)
|
| GSM7906335 |
U2OS cells, non-target library replicate 1 |
| GSM7906336 |
U2OS cells, non-target library replicate 2 |
| GSM7906337 |
U2OS cells, VEGFA site 2 replicate 1 |
|
| This SubSeries is part of SuperSeries: |
| GSE223772 |
Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag |
|
| Relations |
| BioProject |
PRJNA1041818 |
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