 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 29, 2023 |
| Title |
Kir4.1 channels contribute to astrocyte CO2/H+-sensitivity and the drive to breathe |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Astrocytes in the retrotrapezoid nucleus (RTN) stimulate breathing in response to CO2/H+, however, it is not clear how these cells detect changes in CO2/H+. Considering Kir4.1/5.1 channels are CO2/H+-sensitive and important for several astrocyte-dependent processes, we consider Kir4.1/5.1 a leading candidate CO2/H+ sensor in RTN astrocytes. To address this, we show that RTN astrocytes express Kir4.1 and Kir5.1 transcripts. We also characterized respiratory function in astrocyte-specific inducible Kir4.1 knockout mice (Kir4.1 cKO); these mice breathe normally under room air conditions but show a blunted ventilatory response to CO2, which could be partly rescued by viral mediated re-expression of Kir4.1 in RTN astrocytes. At the cellular level, astrocytes in slices from astrocyte-specific inducible Kir4.1 knockout mice are less responsive to CO2/H+ and show a diminished capacity for paracrine modulation of respiratory neurons. These results suggest Kir4.1/5.1 channels in RTN astrocytes contribute to respiratory behavior.
|
| |
|
| Overall design |
Examination of cells from rostral ventrolateral medulla brainslices from 4-OH tamoxifen injected Kir4.1 cKO mice
|
| |
|
| Contributor(s) |
Cleary CM, Mulkey DK |
| Citation(s) |
38548965 |
| |
| Submission date |
Nov 14, 2023 |
| Last update date |
Apr 05, 2024 |
| Contact name |
William F Flynn |
| Organization name |
The Jackson Laboratory
|
| Street address |
10 Discovery Drive
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA1040193 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE247704_RAW.tar |
35.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
