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| Status |
Public on May 03, 2024 |
| Title |
miRNA-mediated inhibition of an actomyosin network in hippocampal pyramidal neurons restricts sociability in adult male mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We recently reported that the loss of the microRNA cluster miR-379-410 leads to hypersocial behavior and anxiety in mice. With this study, we show that ablating miR-379-410 in excitatory neurons of the postnatal mouse hippocampus recapitulates hypersociability, but not anxiety. At the cellular level, miR-379-410 loss in excitatory neurons leads to increased excitatory synaptic transmission and upregulation of an actomyosin gene network. Re-expression of three cluster miRNAs, as well as pharmacological inhibition of the actomyosin activator ROCK, was sufficient to reinstate normal sociability in miR-379-410 knockout mice. Our results unveil a novel microRNA-actomyosin pathway involved in the control of sociability.
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| Overall design |
To investigate whether the knockout of miR379-410 miRNA cluster in glutamatergic neurons of the hippocampus drives differential gene expression, we established genetically modified mice with Cre-driven conditional knockout (cKO) of this cluster in Emx1 positive cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of 6 different animals per genotype (6 wild type and 6 cKO mice).
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| Contributor(s) |
Narayanan R, Germain P, Schratt G |
| Citation(s) |
38968074 |
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| Submission date |
Nov 09, 2023 |
| Last update date |
Aug 02, 2024 |
| Contact name |
Gerhard Schratt |
| E-mail(s) |
gerhard.schratt@hest.ethz.ch
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| Organization name |
ETH Zürich
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| Department |
D-HEST
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| Lab |
Systems Neuroscience
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| Street address |
Winterthurerstrasse 190
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| City |
Zürich |
| ZIP/Postal code |
8057 Zürich |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1037562 |
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