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| Status |
Public on Nov 05, 2023 |
| Title |
LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Small nucleolar RNA host genes (SNHGs) have been implicated in various biological processes, yet their involvement in polycystic ovary syndrome (PCOS) remains elusive. Specifically, SNHG5, a long non-coding RNA implicated in several human cancers, shows elevated expression in granulosa cells (GCs) of PCOS women and induces PCOS-like features when overexpressed in mice. In vitro, SNHG5 inhibits GC proliferation and induces apoptosis and cell cycle arrest at G0/G1 phase, with RNA-seq indicating its impact on DNA replication and repair pathways. Mechanistically, SNHG5 acts as a competing endogenous RNA by binding to miR-92a-3p, leading to increased expression of target gene CDKN1C, which further suppresses GC proliferation and promotes apoptosis. These findings elucidate the crucial role of SNHG5 in the pathogenesis of PCOS and suggest a potential therapeutic target for this condition. Additional investigations such as large-scale clinical studies and functional assays are warranted to validate and expand upon these findings.
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| Overall design |
In order to investigate the biological effects of SNHG5, RNA-seq was performed to analyze the gene expression profiles affected by SNHG5 knockdown in KGN cells.
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| Contributor(s) |
Yang Z, Pan J, Zhou C, Huang H |
| Citation(s) |
38313057 |
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| Submission date |
Nov 03, 2023 |
| Last update date |
Feb 14, 2024 |
| Contact name |
Zuwei Yang |
| E-mail(s) |
yangzuwei7913@fckyy.org.cn
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| Organization name |
Obstetrics and Gynecology Hospital, Fudan University
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| Street address |
No. 419, Fangxie Road
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| City |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1035508 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE246995_FPKM.txt.gz |
398.2 Kb |
(ftp)(http) |
TXT |
| GSE246995_counts.txt.gz |
218.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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