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| Status |
Public on Apr 05, 2011 |
| Title |
mer1D_SK1 meiotic time course on expression/splicing microarrays |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Synchronized mer1D_SK1 meiotic time course.
mer1D diploid was generated in the SK1 strain background (ATCC: MYA-2089) by PCR-mediated cassette-based gene replacement where the MER1 ORF was replaced with HIS3 from S. kluyveri then the transformants were sporulated and the homozygous deletion was verified by PCR.
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| Overall design |
Two independent time courses; Dye swap of each time point; Reference pool WT RNA comprised of 50% time zero RNA plus 10% each of time 2 hours, 5, hours, 7 hours, 9 hours and 11 hours
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| |
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| Contributor(s) |
Trevino L, Munding E |
| Citation(s) |
21123654 |
| |
| Submission date |
Oct 13, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Lily Shiue |
| E-mail(s) |
lshiue@ucsc.edu
|
| Phone |
831-459-2612
|
| Organization name |
UCSC
|
| Department |
MCD Biology
|
| Lab |
Ares
|
| Street address |
1156 High Street
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11043 |
Yeast Splicing/Expression Chip v6.2-6.3 |
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| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE24686 |
Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae |
|
| Relations |
| BioProject |
PRJNA133621 |
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