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| Status |
Public on Nov 02, 2023 |
| Title |
Role of affinity in plasma cell development in the germinal center light zone |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Protective immune responses to many pathogens depend on the development of high affinity antibody-producing plasma cells in germinal centers. Transgenic models suggest that there is a stringent affinity-based barrier to plasma cell development. Whether a similar high affinity barrier regulates plasma cell development under physiologic circumstances, and the nature of the plasma cell fate decision has not been defined precisely. Here we use a fate mapping approach to examine the relationship between germinal center (GC) B cells selected to undergo additional rounds of affinity maturation, germinal center pre-plasma cells and plasma cells. The data show that initial plasma cell selection overlaps with germinal center B cell selection, but that the plasma cell compartment accumulates a less diverse and higher affinity collection of antibodies over time. Thus, whereas the GC continues to diversify over time, affinity-based pre-plasma cell selection sieves the germinal center to enable accumulation of a more restricted group of high affinity antibody secreting plasma cells.
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| Overall design |
c-Myc-GFP or C57Bl6/J male mice were immunized with NP-Ova in Alhydrogel and GC B cells (LZ and DZ) and Plasma cells were FACS-sorted on day 14
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| Contributor(s) |
ElTanbouly M, Ramos V, MacLean A, Chen S, Loewe M, Steinbach S, Ben Tanfous T, Johnson B, Cipolla M, Gazumyan A, Oliveira TY, Nussenzweig MC |
| Citation(s) |
37938344 |
| |
| Submission date |
Oct 26, 2023 |
| Last update date |
Dec 05, 2023 |
| Contact name |
Thiago Y Oliveira |
| E-mail(s) |
toliveira@rockefeller.edu
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| Organization name |
The Rockefeller University
|
| Department |
Immunology, Virology and Microbiology
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| Lab |
Laboratory of Molecular Immunology
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| Street address |
1230 YORK AVE
|
| City |
NEW YORK |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (44)
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| Relations |
| BioProject |
PRJNA1032633 |
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