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Series GSE24637 Query DataSets for GSE24637
Status Public on Dec 01, 2010
Title Genetic and genomic analysis of hyperlipidemia, obesity and diabetes using TALLYHO x C57BL6 F2 mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Type 2 diabetes (T2D) is the most common form of diabetes in humans and is closely associated with dyslipidemia and obesity that magnifies the mortality and morbidity related to T2D. The TALLYHO/JngJ (TH) mouse is a polygenic model for T2D characterized by obesity, hyperinsulinemia, impaired glucose uptake and tolerance, hyperlipidemia, and hyperglycemia. To determine the genetic factors that contribute to these T2D related characteristics in TH mice, we interbred TH mice with C57BL/6J (B6) mice. The parental, F1, and F2 mice were phenotyped at 8, 12, 16, 20, and 24 weeks of age for 4-hour fasting plasma triglyceride, cholesterol, insulin, and glucose levels, as well as body weights. Fat pad and carcass weights were measured at 24 weeks after sacrificing the mice. The F2 mice were genotyped genome-wide for 68 markers. Of 393 genotyped F2 mice, 16 were chosen from the extremes of the triglyceride distribution (8 high and 8 low), and liver, pancreas, muscle and adipose tissue were measured for gene expression. Gene expression quantitative trait locus (eQTL) analysis aided in selection of candidates underlying hyperlipidemia, diabetes and obesity QTLs. We identified several genetic loci that affected quantitative variation in plasma lipid and glucose levels and obesity traits.
Overall design 16 (8 high and 8 low) out of 393 F2 mice were chosen from the extremes of the triglyceride distribution, excluding overtly diabetic mice, and liver, pancreas, muscle and adipose tissue were measured for gene expression. In addition to data from the 64 microarrays on the 16 mice, a supplemental file with phenotypes and marker genotypes is provided for all mice as a supplementary file on the Series record (below). Mouse identification numbers are included to connect the data files.
Contributor(s) Stewart TP, Kim HY, Saxton AM, Kim JH
Citation(s) 21167066
Submission date Oct 12, 2010
Last update date Feb 11, 2019
Contact name Arnold Saxton
Organization name University of Tennessee
Street address Dept. of Animal Science
City Knoxville
State/province TN
ZIP/Postal code 37996
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (64)
GSM607572 Liver, High Triglyceride, Rep 1
GSM607573 Liver, High Triglyceride, Rep 2
GSM607574 Liver, High Triglyceride, Rep 3
BioProject PRJNA132473

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24637_RAW.tar 224.3 Mb (http)(custom) TAR (of CEL)
GSE24637_THxB6-F2-GenoPheno.txt.gz 64.3 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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