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| Status |
Public on Nov 02, 2023 |
| Title |
Inhibiting neddylation with MLN4924 potentiates hypoxia-induced apoptosis of mouse type B spermatogonia GC-2 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hypoxia, an inadequate supply of tissue oxygen tension, has been reported to induce apoptosis of spermatogenic cells and is associated with male infertility. Neddylation, a post-translational modification similar to ubiquitination, has been shown to be involved in the hypoxia stress response. However, the functions of neddylation in hypoxia-induced apoptosis of spermatogenic cells and its association with male infertility remain largely unexplored. In this study, aiming to explore the role of neddylation in male infertility, we used the specific neddylation inhibitor MLN4924 for treatment in mouse type B spermatogonia GC-2 cells. Our results showed that MLN4924 had no apparent effect on GC-2 cell apoptosis under normoxia, but significantly increased apoptotic cells under hypoxia. Transcriptomic analysis and qPCR assay confirmed that MLN4924 could suppress the expression of hypoxia target genes in GC-2 cells under hypoxia. In addition, MLN4924 could enhance the induction of intracellular and mitochondrial reactive oxygen species (ROS) under hypoxia. These results indicate that the neddylation inhibitor MLN4924 potentiates hypoxia-induced apoptosis of mouse type B spermatogonia GC-2 cells, and neddylation may play an important role in promoting spermatogenic cells to adapt to hypoxia stress.
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| Overall design |
GC-2 cells were incubated with MLN4924 (1 μM) or DMSO as control, and then the cells were cultured under normoxia or hypoxia for 24 hours (each sample with 3 biological replicates).
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| Web link |
https://doi.org/10.1016/j.gene.2023.147935
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| Contributor(s) |
Chen Z, Wang Z, Zhu C, Deng H, Chen X |
| Citation missing |
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| Submission date |
Oct 22, 2023 |
| Last update date |
Feb 07, 2024 |
| Contact name |
Hongyan Deng |
| E-mail(s) |
dhy0917@126.com
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| Organization name |
Wuhan University
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| Street address |
299 Bayi Road, Wuchang District
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| City |
Wuhan |
| ZIP/Postal code |
430000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM7852955 |
GC-2 cells, MLN4924 1 μM, hypoxia 24h, 1 [HM1] |
| GSM7852956 |
GC-2 cells, MLN4924 1 μM, hypoxia 24h, 2 [HM2] |
| GSM7852957 |
GC-2 cells, MLN4924 1 μM, hypoxia 24h, 3 [HM3] |
| GSM7852958 |
GC-2 cells, DMSO, normoxia 24h, 1 [ND1] |
| GSM7852959 |
GC-2 cells, DMSO, normoxia 24h, 2 [ND2] |
| GSM7852960 |
GC-2 cells, DMSO, normoxia 24h, 3 [ND3] |
| GSM7852961 |
GC-2 cells, MLN4924 1 μM, normoxia 24h, 1 [NM1] |
| GSM7852962 |
GC-2 cells, MLN4924 1 μM, normoxia 24h, 2 [NM2] |
| GSM7852963 |
GC-2 cells, MLN4924 1 μM, normoxia 24h, 3 [NM3] |
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| Relations |
| BioProject |
PRJNA1030935 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE245989_FPKM_allsample.txt.gz |
512.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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