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| Status |
Public on Feb 21, 2024 |
| Title |
The effect of anti-Gremlin-1 treatment on human cirrhotic precision-cut liver slices |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gremlin-1 has been implicated in liver fibrosis in metabolic dysfunction-associated steatohepatitis (MASH) via inhibition of bone-morphogenetic protein (BMP) signalling and has thereby been identified as a potential therapeutic target. Using human cirrhotic precision-cut liver slices (PCLS) as an ex vivo model systems of MASH fibrosis, we show that neutralisation of Gremlin-1 activity with monoclonal therapeutic antibodies does not reduce liver inflammation or liver fibrosis. Overall, our findings suggest a redundant role for Gremlin-1 in the pathogenesis of liver fibrosis, which is unamenable to therapeutic targeting.
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| |
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for PCLS treated with anti-Gremlin-1 or Isotype control antibodies.
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| |
|
| Contributor(s) |
Horn P, Weston CJ, Newsome PN |
| Citation(s) |
39361025 |
| |
| Submission date |
Oct 22, 2023 |
| Last update date |
Oct 15, 2024 |
| Contact name |
Paul Horn |
| E-mail(s) |
paul.horn@charite.de
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Department |
Department of Hepatology and Gastroenterology
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA1030913 |
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