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| Status |
Public on Jan 10, 2011 |
| Title |
Genomic Collaboration of Estrogen Receptor-α and ERK2 in Regulating Gene and Proliferation Programs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The nuclear hormone receptor, estrogen receptor-alpha (ERα), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERα activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERα colocalization at chromatin binding sites across the genome of breast cancer cells.
KEYWORDS: siRNA knock-down, ligand treatment
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| Overall design |
MCF-7 human breast adenocarcinoma cells were tranfected with control, ERK1 and ERK2 siRNA for 60 hours and treated with 0.1% EtOH (Vehicle) or 10 nM E2 for 4 hours or 24 hours, and cDNA microarray analyses were carried out using Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array.
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| |
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| Contributor(s) |
Erdogan ZM, Katzenellenbogen B |
| Citation(s) |
20956553 |
| |
| Submission date |
Oct 08, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Zeynep Madak Erdogan |
| Organization name |
UIUC
|
| Department |
FSHN
|
| Lab |
Zeynep Madak Erdogan
|
| Street address |
1201 S Goodwin Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (30)
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| Relations |
| BioProject |
PRJNA132379 |
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