|
| Status |
Public on Dec 01, 2023 |
| Title |
A high-throughput screen identifies RNA Binding Proteins that affect fertility in C. elegans and reveals a functional relationship between ADR-2 and SQD-1 |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A high-throughput screen was performed to identify RNA binding proteins that impact fertility in Caenorhabditis elegans hermaphrodites. This screen makes use of animals expressing a vitellogenin (yolk protein) fusion with GFP, in a background (adr-2(-)) that corrects for a previously identified fertility defect in this strain. In addition to identifying several RNA binding proteins that regulate fertility, the screen also uncovered an interaction between the RNA binding proteins adr-2 and sqd-1, which were found to coregulate many transcripts important for fertility.
|
| |
|
| Overall design |
N2 (wildtype) and adr-2(-) animals were hatched and grown for 60 hours on ctrl (empty vector) RNAi or sqd-1 RNAi. Young adult worms were collected and total RNA was isolated from these samples. RNA was poly-A selected before proceeding with library preparation.
|
| |
|
| Contributor(s) |
Erdmann EA, Hundley HA |
| Citation(s) |
37961348 |
| |
| Submission date |
Oct 04, 2023 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Emily Ann Erdmann |
| E-mail(s) |
emierdma@iu.edu
|
| Organization name |
Indiana University
|
| Department |
Biology
|
| Lab |
Hundley
|
| Street address |
1001 E Third Street
|
| City |
BLOOMINGTON |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL32326 |
NextSeq 2000 (Caenorhabditis elegans) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA1023859 |
Less...
More...