|
| Status |
Public on Sep 27, 2023 |
| Title |
Interferon-γ couples CD8+ T cell avidity and differentiation during infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Expression and relevance of IFNg signaling in CD8 T cells during infection
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| |
|
| Overall design |
Expression profiling by high throughput sequencing. For 24hrs experiment, C57 Bl6 mice were left naive or infected with Listeria-OVA infected 24 hrs . CD3+ CD8+ T cells were isolated by FACS from the spleen and subjected to scRNAseq. For day 9 experiment, C57Bl6 WT mice and CD8-IFNgRKO mice were infected with LM-OVA. CD3+ CD8+ OVA-tetramer+ T cells were isolated by Fluorescence-activated cell sorting (FACS) from the spleen after 9 days and subjected to scRNA and scTCR sequencing.
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| |
|
| Contributor(s) |
Gérard A |
| Citation(s) |
37872155 |
| |
| Submission date |
Sep 27, 2023 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Audrey Gerard |
| E-mail(s) |
audrey.gerard@kennedy.ox.ac.uk
|
| Phone |
01865612665
|
| Organization name |
The Kennedy Institute of Rheumatology
|
| Street address |
Roosevelt Dr.
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7FY |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA1021655 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE244203_RAW.tar |
88.8 Mb |
(http)(custom) |
TAR (of CSV, FASTQ, MTX, TSV) |
| GSE244203_d9_KO1_barcodes.tsv.gz |
10.0 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO1_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO1_matrix.mtx.gz |
12.9 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_KO2_barcodes.tsv.gz |
8.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO2_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO2_matrix.mtx.gz |
10.2 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_KO3_barcodes.tsv.gz |
14.5 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO3_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO3_matrix.mtx.gz |
20.8 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_KO4_barcodes.tsv.gz |
17.2 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO4_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_KO4_matrix.mtx.gz |
25.7 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_WT1_barcodes.tsv.gz |
5.8 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT1_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT1_matrix.mtx.gz |
7.6 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_WT2_barcodes.tsv.gz |
5.0 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT2_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT2_matrix.mtx.gz |
6.5 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_WT3_barcodes.tsv.gz |
22.0 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT3_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT3_matrix.mtx.gz |
34.8 Mb |
(ftp)(http) |
MTX |
| GSE244203_d9_WT4_barcodes.tsv.gz |
20.4 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT4_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE244203_d9_WT4_matrix.mtx.gz |
31.2 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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