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| Status |
Public on Jan 11, 2024 |
| Title |
Single cell RNA profiling of neutrophils in a model of pancreatic orthotopic cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Neutrophils are increasingly recognized as key players in the tumour immune response, and are associated with poor clinical outcomes. Despite recent advances characterizing the diversity of neutrophil states in cancer, common trajectories and mechanisms governing the ontogeny and relationship between these neutrophil states remain undefined. Single-cell RNA sequencing (scRNAseq) of neutrophils from the bone marrow, spleen, and blood in addtion to the pancreatic tumor thus uncovers an unique tumor differentiation trajectory, where immature and mature neutrophils converge into a terminally differentiated tumor neutrophil state.
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| Overall design |
CD11b+CD115-Ly6G+ neutrophils were sorted by fluorescence activated cell sorting (FACS) from the bone marrow, spleen, blood and tumor from mice bearing orthotopic tumors previously established from the Pdx1Cre; KrasG12D/+; Trp53R172H/+ (KPC) genetically modified mouse model (n=2). Each sample was independently labelled with TotalSeq-A hashtag antibodies (A0301-A0308), before pooling for 10X v3 3' scRNAseq.
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| Contributor(s) |
Ng MS, Kwok I, Tan L, Shih C, Tan Y, Li M, Ang N, Chen J, Ng L |
| Citation(s) |
38207030 |
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| Submission date |
Sep 18, 2023 |
| Last update date |
Apr 11, 2024 |
| Contact name |
Laiguan Ng |
| E-mail(s) |
Ng_Lai_Guan@immunol.a-star.edu.sg
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| Organization name |
Singapore Immunology Network
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| Street address |
8A Biomedical Grove IMMUNOS bldg, Level 3, SINGAPORE 138648
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| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE244536 |
Deterministic reprogramming of neutrophils in tumors |
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| Relations |
| BioProject |
PRJNA1018542 |
